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In Situ AFM Imaging of Adsorption Kinetics of DPPG Liposomes: A Quantitative Analysis of Surface Roughness

Published online by Cambridge University Press:  28 March 2019

Andreia A. Duarte ,
Joaquim T. Marquês ,
Francisco Brasil ,
Ana S. Viana ,
Pedro Tavares  and
Maria Raposo Open the ORCID record for Maria Raposo [Opens in a new window]
Andreia A. Duarte
Affiliation:
CEFITEC, Departamento de Física, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
Joaquim T. Marquês
Affiliation:
Centro de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Ed. C8, Campo Grande 1749-016, Lisboa, Portugal
Francisco Brasil
Affiliation:
CEFITEC, Departamento de Física, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
Ana S. Viana
Affiliation:
Centro de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Ed. C8, Campo Grande 1749-016, Lisboa, Portugal
Pedro Tavares
Affiliation:
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, UNL, Campus de Caparica, 2829-516 Caparica, Portugal
Maria Raposo*
Affiliation:
CEFITEC, Departamento de Física, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
*
*Author for correspondence: Maria Raposo, E-mail: mfr@fct.unl.pt
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Abstract

The adsorption of intact liposomes on surfaces is of great importance for the development of sensors and drug delivery systems and, also, strongly dependent on the surface roughness where the liposomes are adsorbed. In this paper, we analyzed, by using atomic force microscopy in liquid, the evolution of the morphology of gold surfaces and of poly(allylamine hydrochloride) (PAH) surfaces with different roughness during the adsorption of liposomes prepared with the synthetic phospholipid 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]. Our results reveal the following. On smooth surfaces of Au only and Au with PAH, the liposomes open and deploy on the substrate, creating a supported-lipid bilayer, with the opening process being faster on the Au/PAH surface. On rough substrates of Au coated with polyelectrolyte multilayers, the liposomes were adsorbed intact on the surface. This was corroborated by power spectral density analysis that demonstrates the presence of superstructures with an average lateral size of 43 and 87 nm, in accordance with two and four times the mean liposome hydrodynamic diameter of about 21 nm. In addition, this work presents an adequate and effective methodology for analysis of adsorption phenomena of liposomes on rough surfaces.

Keywords

adsorptionatomic force microscopydynamic scaling analysisliposome
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 25 , Issue 3 , June 2019 , pp. 798 - 809
Copyright
Copyright © Microscopy Society of America 2019 

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