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Micro-Structural Stability of Micropropagated Plants of Vitex negundo L.

Part of: Micrographia Collection

Published online by Cambridge University Press:  16 April 2021

M. Manokari ,
S. Priyadharshini  and
Mahipal S. Shekhawat Open the ORCID record for Mahipal S. Shekhawat [Opens in a new window]
M. Manokari
Affiliation:
Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry605008, India Siddha Clinical Research Unit, Central Council for Research in Siddha, Palayamkottai600106, Tamil Nadu, India
S. Priyadharshini
Affiliation:
Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry605008, India
Mahipal S. Shekhawat*
Affiliation:
Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry605008, India
*
*Author for correspondence: Mahipal S. Shekhawat, E-mail: smahipal3@gmail.com
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Abstract

Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evaluation at different growth conditions allows for understanding structural repair of in vitro raised plantlets. Therefore, the present study was aimed to identify the structural changes that occurred in micropropagated plants of Vitex negundo under heterotrophic, photomixotrophic, and photoautotrophic conditions. To achieve this, structural variations were analyzed in the plantlets obtained from in vitro, greenhouse and field transferred stages using light microscopy. Underdeveloped dermal tissues, palisade cells, intercellular spaces, mechanical tissues, vascular bundles, and ground tissues were observed with the plants growing under in vitro conditions. The self-repairing of structural disorders and transitions in vegetative anatomy was observed during hardening under the greenhouse environment. Field transferred plantlets were characterized by well-developed internal anatomy. These findings showed that the micropropagated plantlets of V. negundo were well-adapted through a series of self-repairing the in vitro induced structural abnormalities at the subsequent stages of plant development.

Keywords

acclimatizationin vitrostructural adaptationsvascular tissues
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 27 , Issue 3 , June 2021 , pp. 626 - 634
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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