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Osmium Tetroxide Labeling of (Poly)Methyl Methacrylate Corrosion Casts for Enhancement of Micro-CT Microvascular Imaging

Published online by Cambridge University Press:  08 October 2013

William L. Mondy ,
Christophe Casteleyn ,
Denis V. Loo ,
Muthkrishna Raja ,
Christopher Singleton  and
Jeffrey G. Jacot
William L. Mondy*
Affiliation:
Division of Congenital Heart Surgery, Texas Children's Hospital, Houston, TX 77030, USA Department of Natural Sciences and Mathematics, Claflin University, Orangeburg, SC 29115, USA Department of Bioengineering, Rice University, Houston, TX 77030, USA
Christophe Casteleyn
Affiliation:
Laboratory for Applied Veterinary Morphology, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium
Denis V. Loo
Affiliation:
UGCT, Department of Physics and Astronomy, Ghent University, Ghent, Belgium
Muthkrishna Raja
Affiliation:
Department of Chemistry, Claflin University, Orangeburg, SC 29115, USA
Christopher Singleton
Affiliation:
Department of Natural Sciences and Mathematics, Claflin University, Orangeburg, SC 29115, USA
Jeffrey G. Jacot
Affiliation:
Division of Congenital Heart Surgery, Texas Children's Hospital, Houston, TX 77030, USA Department of Bioengineering, Rice University, Houston, TX 77030, USA
*
*Corresponding author. E-mail: wmondy@gmail.com
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Abstract

In order to enhance micro-computer tomography (micro-CT) imaging of corrosion casts of fine vasculature, metals can be added to the casting resin before perfusion. However, perfused metals lead to vasoconstriction or vessel damage resulting in nonphysiologic vascular casts. A novel method for coating methyl methacrylate vascular casts with osmium tetroxide has been developed in order to increase micro-CT contrast without affecting the vascular structure. This technique was verified using corrosion casts of the lung vasculature of New Zealand white rabbits. Osmium tetroxide coating of methyl methacrylate vascular corrosion casts resulted in an increase in overall sample contrast that translated into an increase in the resolution of the vasculature. This method can therefore lead to increased resolution in the characterization of fine vascular structures.

Keywords

micro-computer tomographyosmium tetroxidemicrovasculature corrosion casting(poly)methyl methacrylateimage contrastmicrovascular analysis
Type
Biomedical and Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 6 , December 2013 , pp. 1416 - 1427
Copyright
Copyright © Microscopy Society of America 2013 

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