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Quantification of Collagen Organization and Extracellular Matrix Factors within the Healing Ligament

Published online by Cambridge University Press:  13 September 2011

Connie S. Chamberlain ,
Erin M. Crowley ,
Hirohito Kobayashi ,
Kevin W. Eliceiri  and
Ray Vanderby
Connie S. Chamberlain
Affiliation:
Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA
Erin M. Crowley
Affiliation:
Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA
Hirohito Kobayashi
Affiliation:
Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA
Kevin W. Eliceiri
Affiliation:
Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53705, USA Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53705, USA
Ray Vanderby*
Affiliation:
Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53705, USA
*
Corresponding author. E-mail: vanderby@ortho.wisc.edu
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Abstract

Ligament healing of a grade III injury (i.e., a complete tear) involves a multifaceted chain of events that forms a neoligament, which is more scar-like in character than the native tissue. The remodeling process may last months or even years with the injured ligament never fully recovering pre-injury mechanical properties. With tissue engineering and regenerative medicine, understanding the normal healing process in ligament and quantifying it provide a basis to create and assess innovative treatments. Ligament fibroblasts produce a number of extracellular matrix (ECM) components, including collagen types I and III, decorin and fibromodulin. Using a combination of advanced histology, molecular biology, and nonlinear optical imaging approaches, the early ECM events during ligament healing have been better characterized and defined. First, the dynamic changes in ECM factors after injury are shown. Second, the factors associated with creeping substitution are identified. Finally, a method to quantify collagen organization is developed and used. Each ECM factor described herein as well as the temporal quantification of fiber organization helps elucidate the complexity of ligament healing.

Keywords

ligamentmultiphoton microscopyhealingcollagentissue engineering
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 5 , October 2011 , pp. 779 - 787
Copyright
Copyright © Microscopy Society of America 2011

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