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The Redox Dysregulation Visualized by Electron Microscopic Localization of Cerium Perhydroxide in The Kidney of (Nzb X Nzw)F1 Mice.

Published online by Cambridge University Press:  02 July 2020

Megumi Nishikawa ,
Li-Li Chen ,
Rie Igarashi ,
Tomoko Nakazawa  and
Eizo Aikawa
Megumi Nishikawa
Affiliation:
Dept. of Anat. Developmental Biol., 8-1 Kawada-cho, Shinjuku-ku, Tokyo162Japan
Li-Li Chen
Affiliation:
Ophthalmol., Tokyo Women's Medical College, 8-1 Kawada-cho, Shinjuku-ku, Tokyo162Japan
Rie Igarashi
Affiliation:
Inst. of Medical Science, St. Marianna Univ. of Medicine, 2-16-1, Sugau, Miyamae-ku, Kawasaki216Japan
Tomoko Nakazawa
Affiliation:
Dept. of Anat. Developmental Biol., 8-1 Kawada-cho, Shinjuku-ku, Tokyo162Japan
Eizo Aikawa
Affiliation:
Dept. of Anat. Developmental Biol., 8-1 Kawada-cho, Shinjuku-ku, Tokyo162Japan
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Extract

Redox dysregulation is known as pathogenesis of renal involvement in autoimmune lupus mice [1,2]. However, the behavior of reactive oxygen species (ROS) is still unclear [3]. The purpose of this study is to make clear the localization of hydrogen perhydroxide which is generated as intermediate substances of radical reactions.

Modified Brigg's method [4] was used to determine the generation of ROS in renal tissues of (NZB x NZW)F1 mice. The renal specimens were incubated with a standard medium, which consisted of 0. IM tris-malate buffer (pH7.4) with 7% sucrose, lmM CeCl3 and lOmM aminotriazole at 37° C for 30 min and ultrathin sections were prepared and examined by electron microscopy. Lecithinized-recombinant-superoxide dismutase (SOD) was used as redox regulator, which is ROS inhibitor. SOD (1000 U/kg) was injected 3 times a week, tail-intravenously to Fl mice from 4 to 60 weeks of age. The mRNA of inflammatory cytokines and IkB was detected by RT-PCR method.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 59 - 60
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Shah, S.V.et al.,Kidney Int. 35(1989)1093.10.1038/ki.1989.96CrossRefGoogle Scholar
2.Johnson, R.J.et al., Wilson Brenner Stem (1991)87.Google Scholar
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