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Regulation of Peripheral Spindle Movement and Spindle Rotation during Mouse Oocyte Meiosis: New Perspectives

Published online by Cambridge University Press:  04 July 2008

Jun-Shu Ai ,
Qiang Wang ,
Shen Yin ,
Li-Hong Shi ,
Bo Xiong ,
Ying-Chun OuYang ,
Yi Hou ,
Da-Yuan Chen ,
Heide Schatten  and
Qing-Yuan Sun
Jun-Shu Ai
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Graduate School, Chinese Academy of Sciences, Beijing 100101, China
Qiang Wang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Graduate School, Chinese Academy of Sciences, Beijing 100101, China
Shen Yin
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Graduate School, Chinese Academy of Sciences, Beijing 100101, China
Li-Hong Shi
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Graduate School, Chinese Academy of Sciences, Beijing 100101, China
Bo Xiong
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Graduate School, Chinese Academy of Sciences, Beijing 100101, China
Ying-Chun OuYang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Yi Hou
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Da-Yuan Chen
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Heide Schatten
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO 65211, USA
Qing-Yuan Sun*
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
*
Corresponding author. E-mail: sunqy@ioz.ac.cn
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Abstract

Spindle movement, including spindle migration during first meiosis and spindle rotation during second meiosis, is essential for asymmetric divisions in mouse oocytes. Previous studies by others and us have shown that microfilaments are required for both spindle migration and rotation. In the present study, we aimed to further investigate the mechanism controlling spindle movement during mouse oocyte meiosis. By employing drug treatment and immunofluorescence microscopy, we showed that dynamic microtubule assembly was involved in both spindle migration and rotation. Furthermore, we found that the calcium/CaM/CaMKII pathway was important for regulating spindle rotation.

Keywords

mouse oocytespindle migrationspindle rotationmicrotubulecalcium
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 14 , Issue 4 , August 2008 , pp. 349 - 356
Copyright
Copyright © Microscopy Society of America 2008

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References

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