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Shear and Substrate Dependent Changes in Fibrinogen and Von Willebrand Factor Studied by Atomic Force Microscopy

Published online by Cambridge University Press:  02 July 2020

Roger E. Marchant ,
P. Sidney Sit ,
Madhusudan Raghavachari  and
Christopher A. Siedlecki
Roger E. Marchant
Affiliation:
Departments of Biomedical Engineering, Cleveland, OH, 44106 Physiology and Biophysics Case Western Reserve University, Cleveland, OH, 44106
P. Sidney Sit
Affiliation:
Departments of Biomedical Engineering, Cleveland, OH, 44106
Madhusudan Raghavachari
Affiliation:
Departments of Biomedical Engineering, Cleveland, OH, 44106
Christopher A. Siedlecki
Affiliation:
Departments of Biomedical Engineering, Cleveland, OH, 44106
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Extract

Atomic force microscopy (AFM) provides unique opportunities to study cell-surface and molecular scale interactions in three dimensions under aqueous conditions. Two plasma proteins, von Willebrand Factor (vWf) and fibrinogen, play central roles in the regulation of hemostasis and thrombosis by participating in coagulation and facilitating adhesion and aggregation of activated platelets. vWf and fibrinogen are believed to facilitate platelet adhesion under regions of relatively high and low vascular wall shear stress, respectively. Consequently, elucidating vWf and fibrinogen structure-function relations under shear is of considerable importance in developing a comprehensive understanding of the pathophysiology of thrombogenesis. Previously, we reported on molecular level AFM images of human vWf during shear-induced structural transition and human fibrinogen under aqueous conditions when both proteins were adsorbed on a hydrophobic surface. This presentation will report on the shear dependent interactions of dimeric and multimeric vWf with a hydrophobic surface and the substrate-dependent interactions of fibrinogen.

Type
Biomaterials
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 924 - 925
Copyright
Copyright © Microscopy Society of America

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References

1.Siedlecki, C.A. and Marchant, R.E.. Biomaterials (in press).Google Scholar
2.Siedlecki, C.A., et al. (1996) Blood. 88:2939.CrossRefGoogle Scholar
3.Marchant, R.E., et al. (1997) Thrombosis and Haemostasis. 77:1048.Google Scholar
4. The authors gratefully acknowledge use of the facilities of the Center for Cardiovascular Biomaterials, and financial support from NIH grant HL-40047 and HL-58540, and the American Heart Association.Google Scholar