Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-13T02:38:56.847Z Has data issue: false hasContentIssue false
  • English
  • Français

Short-Term Response of Mitomycin C on the Human Rectus Muscle Following Strabismus Surgery: Histological, Ultrastructural, and Biomechanical Evaluation

Published online by Cambridge University Press:  04 February 2013

Samjin Choi ,
Youjin Cheong ,
Jae-Ho Shin ,
Kyung A Kim ,
Jae Beum Bang ,
Kyung-Hyun Jin  and
Hun-Kuk Park
Samjin Choi
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea Department of Orthodontics, Kyung Hee University, Seoul, Korea
Youjin Cheong
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea
Jae-Ho Shin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul, Korea
Kyung A Kim
Affiliation:
Department of Orthodontics, Kyung Hee University, Seoul, Korea
Jae Beum Bang
Affiliation:
Department of Dental Education, Kyung Hee University, Seoul, Korea
Kyung-Hyun Jin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul, Korea
Hun-Kuk Park*
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea Department of Medical Engineering, Kyung Hee University, Seoul, Korea
*
*Corresponding author. E-mail: sigmoidus@khu.ac.kr
Get access

Abstract

This study investigated the inflammatory effect of intraoperative mitomycin C (MMC) on adhesion reformation in human rectus muscles. Ten consecutive patients who underwent medial rectus resection had their postoperative rectus muscles divided into two groups: control group (n = 10) and MMC group (n = 10). In the MMC group, the muscle was soaked for 2 min with MMC, prepared as a 0.2 mg/mL (0.02%) solution. The 0.02% MMC reactions were examined using histological analysis with hematoxylin-eosin (inflammatory response) and Masson's trichrome (collagen fibrils), immunoreactivities of cyclooxygenase-II (inflammatory response), and collagen type I and III, scanning electron microscopy analysis to quantify the diameter and D-periodicity of collagen fibrils, and atomic force microscopy analysis to quantify the diameter, D-periodicity, and adhesion force of collagen fibrils. The rectus muscles treated with 0.02% MMC showed a significantly increased inflammatory response (p < 0.05), increased collagen density (p < 0.0001), increased fibril diameter (p < 0.001 or p < 0.05), and decreased fibril adhesion force (p < 0.005) compared to the rectus muscles in the control group. MMC simultaneously caused an inflammatory response as well as nanostructural and biomechanical property changes in the collagen fibril network.

Keywords

mitomycin C (MMC)human rectus musclescollagen fibrilsnanostructural and biomechanical propertiesinflammation
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 1 , February 2013 , pp. 227 - 232
Copyright
Copyright © Microscopy Society of America 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anduze, A.L. & Burnett, J.M. (1996). Indications for and complications of mitomycin-C in pterygium surgery. Ophthalmic Surg Lasers 27, 667–673.Google Scholar
Brooks, S.E., Ribeiro, G.B., Archer, S.M., Elner, V.M. & Del Monte, M.A. (1996). Fat adherence syndrome treated with intraoperative mitomycin-C: A rabbit model. J Pediatr Ophthalmol Strabismus 33, 21–27.Google Scholar
Cengiz, Y., Altintas, O., Manav Ay, G. & Cağlar, Y. (2005). Comparison of the effectiveness of mitomycin-C and Viscoat on delayed adjustable strabismus surgery in rabbits. Eur J Ophthalmol 15, 530–535.Google Scholar
Chen, P.L., Chen, W.Y. & Lu, D.W. (2005). Evaluation of mitomycin C in reducing postoperative adhesions in strabismus surgery. J Ocul Pharmacol Ther 21, 406–410.Google Scholar
Choi, H.Y., Lee, J.H., Lee, J.E. & Jung, J.H. (2010). Effect of bevacizumab on strabismus surgery in rabbits. Invest Ophthalmol Vis Sci 51, 4585–4588.Google Scholar
Choi, S., Lee, S.J., Shin, J.H., Cheong, Y., Lee, H.J., Paek, J.H., Kim, J.S., Jin, K.H. & Park, H.K. (2011). Ultrastructural investigation of intact orbital implant surfaces using atomic force microscopy. Scanning 33, 211–221.Google Scholar
Majmudar, P.A., Forstot, S.L., Dennis, R.F., Nirankari, V.S., Damiano, R.E., Brenart, R. & Epstein, R.J. (2000). Topical mitomycin-C for subepithelial fibrosis after refractive corneal surgery. Ophthalmology 107, 89–94.Google Scholar
Mamede, F.V., Laus, J.L., Cabral, V.P., Vicenti, F.A. & Barbieri-Neto, J. (2004). Use of topical mitomycin C in myoplasty of the medial rectus muscle of rabbits. Vet Ophthalmol 7, 335–342.Google Scholar
Minguini, N., Monteiro de Carvalho, K.M., Akaishi, P.M. & De Luca, I.M. (2000). Histologic effect of mitomycin C on strabismus surgery in the rabbit. Invest Ophthalmol Vis Sci 41, 3399–3401.Google Scholar
Wan Norliza, W.M., Raihan, I.S., Azwa, J.A. & Ibrahim, M. (2006). Scleral melting 16 years after pterygium excision with topical mitomycin C adjuvant therapy. Cont Lens Anterior Eye 29, 165–167.Google Scholar
Yan, L., Sun, Y., Wang, J., Dai, S., Feng, X., Jiang, B., Wang, Q., Yu, T., Shi, X. & Yang, J. (2010). The effect of mitomycin C in reducing intraarticular adhesion after knee surgery in rabbits. Eur J Pharmacol 643, 1–5.Google Scholar