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Subcellular Localization of a Novel Alternative Splicing of IIIG9 and Colocalization with PPP1gamma Isoforms

Published online by Cambridge University Press:  03 October 2008

C. Sousa ,
A.P. Vintém ,
M. Fardilha ,
O. da Cruz e Silva  and
E. da Cruz e Silva
C. Sousa*
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
A.P. Vintém
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
M. Fardilha
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
O. da Cruz e Silva
Affiliation:
Neurosciences Lab, CBC, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
E. da Cruz e Silva
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
*
Corresponding author.

Abstract

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In testis we find mainly PPP1gamma2 isoform. We hypothesize that in different cell types we can find different regulatory subunits that may constitute targets for therapeutics of diseases such as male infertility, cancer and Alzheimer's disease. We identified a novel alternative splicing isoform of IIIG9 in testis, a known regulator of PPP1, IIIG9sT, and the aim of this study was its further characterization. We used a specific antibody for IIIG9sT in order to characterize its localization in bovine sperm cells. We also transfected IIIG9sT-GFP construct in mouse spermatogonia cells (GC-1 cells) and we used specific antibodies for each PPP1 isoform for the colocalization studies. We observed them under a fluorescent microscope and a LSM and quantified a high co-localization with PPP1gamma1 and 2 isoforms.

Type
Abstract
Information
Microscopy and Microanalysis , Volume 14 , Supplement S3 , September 2008 , pp. 141 - 143
Copyright
Copyright © Microscopy Society of America 2008