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Surface Microanalysis and Chemical Imaging of Early Dentin Remineralization

Published online by Cambridge University Press:  25 October 2013

Manuel Toledano ,
Inmaculada Cabello ,
Miguel Angel Cabrerizo Vílchez ,
Miguel Angel Fernández  and
Raquel Osorio
Manuel Toledano*
Affiliation:
Faculty of Dentistry, University of Granada, Dental Materials Section, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
Inmaculada Cabello
Affiliation:
Faculty of Dentistry, University of Granada, Dental Materials Section, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
Miguel Angel Cabrerizo Vílchez
Affiliation:
Faculty of Sciences, University of Granada, Applied Physics Section, Campus de Fuentenueva s/n, 18071 Granada, Spain
Miguel Angel Fernández
Affiliation:
Faculty of Sciences, University of Granada, Applied Physics Section, Campus de Fuentenueva s/n, 18071 Granada, Spain
Raquel Osorio
Affiliation:
Faculty of Dentistry, University of Granada, Dental Materials Section, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
*
*Corresponding author. E-mail: toledano@ugr.es
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Abstract

This study reports physical and chemical changes that occur at early dentin remineralization stages. Extracted human third molars were sectioned to obtain dentin discs. After polishing the dentin surfaces, three groups were established: (1) untreated dentin (UD), (2) 37% phosphoric acid application for 15 s (partially demineralized dentin—PDD), and (3) 10% phosphoric acid for 12 h at 25° C (totally demineralized dentin—TDD). Five different remineralizing solutions were used: chlorhexidine (CHX), artificial saliva (AS), phosphate solution (PS), ZnCl2, and ZnO. Wettability (contact angle), ζ potential and Raman spectroscopy analysis were determined on dentin surfaces. Demineralization of dentin resulted in a higher contact angle. Wettability decreased after immersion in all solutions. ζ potential analysis showed dissimilar performance ranging from −6.21 mV (TDD + AS) up to 3.02 mV (PDD + PS). Raman analysis showed an increase in mineral components after immersing the dentin specimens, in terms of crystallinity, mineral content, and concentration. This confirmed the optimal incorporation and deposition of mineral on dentin collagen. Organic content reflected scarce changes, except in TDD that appeared partially denatured. Pyridinium, as an expression of cross-linking, appeared in all spectra except in specimens immersed in PS.

Keywords

Ramancontact angleζ potentialdentindemineralizationremineralization
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 1 , February 2014 , pp. 245 - 256
Copyright
Copyright © Microscopy Society of America 2014 

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