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Time-resolved CryoEM Using Spotiton

Published online by Cambridge University Press:  30 July 2020

William Budell ,
Venkata Dandey ,
Hui Wei ,
Daija Bobe ,
Kashyap Maruthi ,
Mykhailo Kopylov ,
Edward Eng ,
Peter Kahn ,
Clint Potter  and
Bridget Carragher
William Budell
Affiliation:
New York Structural Biology Center, New York, New York, United States
Venkata Dandey
Affiliation:
New York Structural Biology Center, New York, New York, United States
Hui Wei
Affiliation:
New York Structural Biology Center, New York, New York, United States
Daija Bobe
Affiliation:
New York Structural Biology Center, New York, New York, United States
Kashyap Maruthi
Affiliation:
New York Structural Biology Center, New York, New York, United States
Mykhailo Kopylov
Affiliation:
New York Structural Biology Center, New York, New York, United States
Edward Eng
Affiliation:
New York Structural Biology Center, New York, New York, United States
Peter Kahn
Affiliation:
Engineering Arts, LLC, Tempe, Arizona, United States
Clint Potter
Affiliation:
New York Structural Biology Center, New York, New York, United States
Bridget Carragher
Affiliation:
New York Structural Biology Center, New York, New York, United States

Abstract

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Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 326 - 327
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Copyright
Copyright © Microscopy Society of America 2020

References

Jain, et al. Spotiton: a prototype for an integrated inkjet dispense and vitrification system for cryo-TEM. J Struct Biol. 179, 68–75. 2012 10.1016/j.jsb.2012.04.020CrossRefGoogle ScholarPubMed
Razinkov, et al. A new method for vitrifying samples for cryoEM. J Struct Biol. 195, 190–8. 2016.10.1016/j.jsb.2016.06.001CrossRefGoogle ScholarPubMed
Dandey, et al. Spotiton: New features and applications. J Struct Biol. 202, 161–169. 2018.10.1016/j.jsb.2018.01.002CrossRefGoogle ScholarPubMed
Wei, et al. Optimizing “self-wicking” nanowire grids. J Struct Biol. 202, 170–174. 0218.10.1016/j.jsb.2018.01.001CrossRefGoogle Scholar
Noble, et al. Reducing effects of particle adsorption to the air-water interface in cryoEM. Nat Methods 15, 793–795. 2018.10.1038/s41592-018-0139-3CrossRefGoogle Scholar
Funding support from the Simons Foundation (SF349247) and the NIH (GM103310).Google Scholar