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Towards Understanding the Structural Requirements for Endogenous Transcription in Rotavirus

Published online by Cambridge University Press:  02 July 2020

J. A. Lawton ,
M. K. Estes  and
B. V. Venkataram Prasad
J. A. Lawton
Affiliation:
Program in Cell and Molecular Biology, Houston, TX77030.
M. K. Estes
Affiliation:
Division of Molecular Virology, Baylor College of Medicine, Houston, TX77030.
B. V. Venkataram Prasad
Affiliation:
Department of Biochemistry, Houston, TX77030.
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Extract

Rotaviruses are complex, relatively large (1000 Å) nonenveloped icosahedral viruses. They are the major causative agents of severe diarrhea in children and animals. Annually an estimated 1 million children die from rotavirus-induced gastroenteritis worldwide. Medical relevance, intriguing structural complexity, and several unique strategies in the morphogenesis and replication have provoked extensive molecular biological and structural studies on rotaviruses in recent years.

Rotaviruses are double-stranded RNA (dsRNA) viruses belonging to the family Reoviridae. Each of the 11 segments codes for one protein. Of the eleven proteins encoded by the genomic RNA, six are structural and five are non-structural. Structural studies have shown that mature rotaviruses are triple-layered particles (TLPs). During the process of cell entry the outer-most layer consisting of VP7 and the spike protein VP4 is lost and the resulting double layered particles (DLPs) become transcriptionally active (Fig. 1 A). During the process of transcription, the DLPs maintain their structural Integrity.

Type
Application of Classical and Novel Microscopy to Tissue Injury and Infectious Disease Pathogenesis
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1050 - 1051
Copyright
Copyright © Microscopy Society of America

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References

1. Prasad, B.V.V. and Estes, M.K., in Chiu et al., Eds., Structural Biology of Viruses, New York: Oxford (1996) 239.Google Scholar

2. Prasad, B.V.V. et al., Nature 382 (1996)471.CrossRefGoogle Scholar

3. Lawton, J. A. et al., Nature Struct. Biol. 4 (1977)118.CrossRefGoogle Scholar

4. We acknowledge Dr. Richard Ward for a generous gift of anti-VP6 ascites, and support from NIH grants Al 36040 (BVVP), DK 30144 (MKE), and a training fellowship to JAL (GM-08280).Google Scholar