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Tubule Density and Diameter in Coronal Dentin from Primary and Permanent Human Teeth

Published online by Cambridge University Press:  15 August 2013

Tathiane L. Lenzi ,
Camila de Almeida B. Guglielmi ,
Victor E. Arana-Chavez  and
Daniela P. Raggio
Tathiane L. Lenzi
Affiliation:
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Lineu Prestes, 2227, Cidade Universitária, São Paulo 05508-000, Brazil
Camila de Almeida B. Guglielmi
Affiliation:
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Lineu Prestes, 2227, Cidade Universitária, São Paulo 05508-000, Brazil
Victor E. Arana-Chavez
Affiliation:
Department of Biomaterials and Oral Biology, School of Dentistry, Universidade de São Paulo, Av. Lineu Prestes, 2227, Cidade Universitária, São Paulo 05508-000, Brazil
Daniela P. Raggio*
Affiliation:
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Lineu Prestes, 2227, Cidade Universitária, São Paulo 05508-000, Brazil
*
*Corresponding author. E-mail: danielar@usp.br
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Abstract

This study compared dentinal tubule density and diameter of human primary and permanent teeth at different depths of the coronal dentin. Crowns of eight primary second molars and eight permanent third molars were serially sectioned into three disks of ~0.5 mm thickness (superficial, middle, and deep layers), perpendicular to the long axis. Tubule density and diameter were evaluated in 2,000× and 3,000× magnifications by scanning electron microscopy. Data obtained were subjected to two-way repeated measures ANOVA and Tukey's post hoc test (α = 0.05). Tubule density was greater in primary teeth compared with permanent ones, regardless of depth (primary: 124,329 ± 43,594 mm2; permanent: 45,972 ± 21,098 mm2). In general, the tubule density increased as the dentin depth increased, except to the superficial and middle layers from permanent teeth. Tubule diameter was larger in the dentin layer close to the pulp chamber (superficial: 2.4 ± 0.07 μm; middle: 3.70 ± 0.06 μm; deep: 4.28 ± 0.04 μm). No difference was observed between primary (3.48 ± 0.81 μm) and permanent teeth (3.47 ± 0.73 μm). The tubule diameter increases as the dentin depth increases for primary and permanent teeth; however, the tubule density is higher in primary teeth.

Keywords

dentinhuman toothmicrostructurescanning electron microscopy
Type
Biomedical and Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 6 , December 2013 , pp. 1445 - 1449
Copyright
Copyright © Microscopy Society of America 2013 

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