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Ultrastructural Analysis of In Vivo Hypoglycemiant Effect of Two Polyoxometalates in Rats with Streptozotocin-Induced Diabetes

Published online by Cambridge University Press:  07 September 2015

Ştefana Bâlici ,
Modeste Wankeu-Nya ,
Dan Rusu ,
Gheorghe Z. Nicula ,
Mariana Rusu ,
Adrian Florea  and
Horea Matei
Ştefana Bâlici
Affiliation:
Department of Cell and Molecular Biology, Faculty of Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur St., 400349 Cluj-Napoca, România Department of Inorganic Chemistry, Faculty of Chemistry and Chemical Engineering, “Babeş-Bolyai” University, 11 Arany Janos St., 400028 Cluj-Napoca, România
Modeste Wankeu-Nya
Affiliation:
Department of Cell and Molecular Biology, Faculty of Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur St., 400349 Cluj-Napoca, România Laboratory of Animal Biology and Physiology, Department of Animal Organism Biology, Faculty of Science, University of Douala, PO Box 24157, Douala, Cameroon
Dan Rusu
Affiliation:
Department of Physical-Chemistry, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur St., 400349 Cluj-Napoca, România
Gheorghe Z. Nicula
Affiliation:
Department of Cell and Molecular Biology, Faculty of Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur St., 400349 Cluj-Napoca, România
Mariana Rusu
Affiliation:
Department of Inorganic Chemistry, Faculty of Chemistry and Chemical Engineering, “Babeş-Bolyai” University, 11 Arany Janos St., 400028 Cluj-Napoca, România
Adrian Florea*
Affiliation:
Department of Cell and Molecular Biology, Faculty of Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur St., 400349 Cluj-Napoca, România
Horea Matei
Affiliation:
Department of Cell and Molecular Biology, Faculty of Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur St., 400349 Cluj-Napoca, România
*
*Corresponding author. aflorea@umfcluj.ro
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Abstract

Two polyoxometalates (POMs), synthesized through a self-assembling method, were used in the treatment of streptozotocin (STZ)-induced diabetic rats. One of these nanocompounds [tris(vanadyl)-substituted tungsto-antimonate(III)-anions—POM1] was previously described in the literature, whereas the second [tris-butyltin-21-tungsto-9-antimonate(III)-anions—POM2], was prepared by us based on our original formula. In rats with STZ-induced diabetes treated with POMs (up to a cumulative dose of 4 mg/kg bodyweight at the end of the treatments), statistically significant reduced levels of blood glucose were measured after 3 weeks, as compared with the diabetic control groups (DCGs). Ultrastructural analysis of pancreatic β-cells (including the mean diameter of secretory vesicles and of their insulin granules) in the treated diabetic rats proved the POMs contribute to limitation of cellular degeneration triggered by STZ, as well as to the presence of increased amounts of insulin-containing vesicles as compared with the DCG. The two POMs also showed hepatoprotective properties when ultrastructural aspects of hepatocytes in the experimental groups of rats were studied. Based on our in vivo studies, we concluded that the two POMs tested achieved hypoglycemiant effects by preventing STZ-triggered apoptosis of pancreatic β-cells and stimulation of insulin synthesis.

Keywords

diabetespolyoxometalatespancreatic β-cellstransmission electron microscopyinsulin
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 5 , October 2015 , pp. 1236 - 1248
Copyright
© Microscopy Society of America 2015 

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