Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-27T07:25:40.908Z Has data issue: false hasContentIssue false
  • English
  • Français

Artifacts from Rapid Microwave Processing of Trematode Tissues (Ascocotyle pachycystis and leighi)

Published online by Cambridge University Press:  14 March 2018

Mark H. Armitage
Mark H. Armitage*
Affiliation:
Liberty University, Thousand Oaks, CA
*
mharmitage@liberty.edu

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The use of microwave energy to assist in the processing of biological tissues for microscopy has generated significant interest in recent years. Microwave (MW) processing has been used to prepare tissues for light microscopy (Carranza et al. 1990 [using parasite tissues]; van Dorp et al. 1995; Davis et al. 1997; Izumi et al 2000; and Rohr et al. 2001), as well as for electron microscopy (Kasa et al. 1982; Hopwood et al. 1984; Leong et al. 1985; Kang et al, 1991 [using parasite tissues]; Heumann 1992; Wagenaar et al. 1993; Login and Dvorak 1993; Giberson and Demaree 1995; Madden and Miriam 1997; Giberson et al. 1997; Morin et al. 1997; Petrali and Mills 1999; Massa and Arana-Chavez 2000; Hernandez and Guillen 2000 [using parasite tissues]; Demaree 2001; Giberson 2001).

Most reports, particularly those published by manufacturers of microwave ovens, have shown positive results regarding tissue ultrastructure, however discussion continues on possible mechanisms of preservation by the use of MW technology.

Type
Research Article
Information
Microscopy Today , Volume 12 , Issue 5 , September 2004 , pp. 32 - 35
Copyright
Copyright © Microscopy Society of America 2004

References

Literature cited

Armitage, M (2000) Ultrastructure of metacercarial cysts of six heterophyid trematodesfrom fish. Parasitol Res 86: 1003–1007 CrossRefGoogle ScholarPubMed
Carranza, GA, Cruz, D, Wagner, GG (1990) Microwave fixation: in situ tick [Ascari: Ixodidae) histoanatomy, ihin sectioning of tick tissues and antigen preservation in mouse spleen. J Med Ent 27(6): 1067–1070 Google Scholar
Davis, C, Neill, S, Raj, P (1997) microwave fixation of rabies specimens for fluorescent antibody testing. I Virol Methods 68(2):177–182 CrossRefGoogle ScholarPubMed
Demaree, RS (2001) Microwave tissue processing: history and SEM techniques. Proc Micro Microanal 7:1190–1191 CrossRefGoogle Scholar
Galve, JJ, Giberson, RT, Cardiff, RD (2004) Microwave mechanisms; the energy/heal dichotomy. Microscopy Today 12(2):18–23 Google Scholar
Giberson, RT, (2001) Advances in microwave assisted processing for electron microscopy. Proc Micro Microanal 7:1192–1193 Google Scholar
Giberson, RT, Demaree, RS (1995) Microwave fixation: understanding the variables to achieve rapid reproducible results. Micros Res Tech 32(3):246–254 Google Scholar
Giberson, RT, Demaree, RS, Nordhausen, RW (1997) Four hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J Vet Diag Invest 9:61–67 Google Scholar
Hernandez, F, Guillen, R (2000) Microwave processing for scanning electron microscopy. Eur J Morpho 38(2):109–l11 Google Scholar
Heumann, HG (1992) Microwave stimulated glutaraldehyde and osmium tetroxide fixation of plant tissue: ultra structural preservation in seconds. Histnchern 97(4):341–347 Google Scholar
Hopwood, D. Coghill, G, Ramsay, J, Kerr, M (1984) Microwave fixation: its potential for routine techniques, hislochemistry, immunocytochemistry, and electron microscopy. Histochem 116(11):1171–1192 Google Scholar
Izumi, Y, Hammerman, SB, Benz, AM, Labruyere, J, Zormuski, CF, Olney, JW (2000) Comparison of rat retinal fixation techniques: chemical fixation and microwave irradiation. Exp Eye Res 70(2):191–198 Google Scholar
Kang, Z, Rob, ringer R, Chong, J, Haber, S (1991) Microwave fixation of rust infected wheat leaves: preservation of fine structure and detection of cell surface antigens, lectin and sugar binding sites. Protoplasms 162(1):27–37 CrossRefGoogle Scholar
Kasa, P. Bansaghy, K., Gulya, K (1982) Ultrastructural changes in diffusion of acetylcholine in rat brain after microwave irradiation. J Neurosci Methods 5(3):215–220 Google Scholar
Leong, AS, Daymon, ME, Milios, J (1985) Microwave irradiation as a form of fixation for light and electron microscopy. J Path 146(4):313–321 Google Scholar
Login, GR, Dvorak, AM (1993) A review of rapid microwave fixation technology: its expanding niche in morphological studies. Scanning l5(2):58–66 Google Scholar
Lumsden, RD (1968) Ultrastructure of the metacercarial cyst of Ascocolylechandleri. Lumsden, 1963 (Trematoda:heterophyidae). Helminth Soc Wash 35(2):212–219 Google Scholar
Madden, VJ, Miriam, MH (1997) Rapid decalcification of temporal bones with preservation of ultrastructure. Hear Res 111:76–84 Google Scholar
Massa, LF, Arana-Chavez, VE (2000) Ultrastructural preservation of rat, embryonic dental tissues after rapid fixation and dehydration under microwave irradiation. Eur J Oral Sci 108(1):74–77 Google Scholar
Morin, F, Crevier, C, Bouvier, G, Lacaille, J-C, Beaulieu, C (1997) A fixation procedure for ultras!ructural investigation of synaptic connections in resected human cortex. Brain Res Bull 44(2):205–210 CrossRefGoogle Scholar
Petrali, JP, Mills, KR (1999) Microwave assisted immunoelectron microscopy of skin: localization of laminin, type IV collagen and btillous pemphigoid antigen, J. Toxicol; Cut Ocul Toxicol 18(4):341–348 Google Scholar
Rohr, LR, Layfield, LJ, Wallin, D, Hardy, D (2001) A comparison of routine and rapid microwave tissue processing in a surgical pathology laboratory: quality of histologic sections and advantages of microwave processing. Am I Clin Path 115(5):703–708 Google Scholar
van Dorp, R. Boon, ME, Kok, PG, Marani, E (1995) Combining microwave stabilization and microwave stimulated fixation of brain tissue with microwave stimulated staining. Eur J Morpho 33(2):164–173 Google ScholarPubMed
Wagenaar, F, Kok, GL, Broekhu risen-Davies JM, Pol JM (1993) Rapid cold fixation of tissue samples by microwave irradiation for use in electron microscopy. Histochem j 25(10):719–725 Google Scholar