Calibration Of Electron Microscopes: How To Do This, How Often, Pit-falls And Problems. M&M 2001 Experts' Session on Core Facility Management

Extract

This article is the third of a series transcribed from the discussion taped during the Core Facility Management session. Buileted paragraphs indicate comments by individuals attending the session.

D. Sherman: The next topic we are going to deal with has to do with calibration of electron microscopes. This became very important in my situation because we started dealing with people with nanotubes and nanovesicles and with very small things where they needed to have accurate size both in the SEM and the TEM magnification range. My own frustrations dealing with calibration, especially in the in between magnification, spurred me on to ask a couple of experts to give us some insight on what we should be doing with calibration - the problems associated with this and some general information about how to go about doing this in our standard facilities where money is short.