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The Champagne Artifact in Negative Staining TEM

Published online by Cambridge University Press:  14 March 2018

John J. Bozzola
John J. Bozzola*
Affiliation:
Southern Illinois University

Extract

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Negative staining is a simple yet extremely useful procedure for examining nanometer-sized specimens such as intact microorganisms (viruses and some bacteria), subcellular components, and even nonbidogical particulates. It is a well established procedure, with extensive literature in many disciplines (Hayat and Miller, 1990). Although numerous variations exist, the basic procedure involves placing the specimen and stain onto a grid containing a substrate - usually plastic with or without a carbon backing. The stains consist of salts of heavy metals such as tungsten, uranium, or molybdenum which surround and often penetrate the specimen. Afier drying into an amorphous, glass-like background, the stains provide contrast based upon differential thickness.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 10 , December 1998 , pp. 24 - 25
Copyright
Copyright © Microscopy Society of America 1998

References

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