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Choosing Between Confocal Microscopes : A Fly-Eye's View

Published online by Cambridge University Press:  14 March 2018

Mark. H.C. Lam*
Affiliation:
John Curtin School of Medical Research, Canberra City, Australia
David A. Jans
Affiliation:
John Curtin School of Medical Research, Canberra City, Australia

Extract

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We have recently been shopping for a confocal microscope and have found a simple way to test the capabilities of the various units that have been demonstrated to us. Some of the important aspects that we were looking for in our “ideal” multi-user system were:

  1. 1) Ease of use.

  2. 2) The ability to obtain good X-Y, X-Z and hence X-Y-Z resolution, and the accurate display of these images.

  3. 3) The depth within the sample at which the system can resolve structures (this of course depends mostly on the lenses used), but is still an important criteria as it reflects on the system as a whole.

  4. 4) “extras” such as a transmission detector and reconstruction/ analysis/image manipulation software.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2000