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Confocal Microscopy System Performance: Field Illumination

Published online by Cambridge University Press:  14 March 2018

Robert M. Zucker
Robert M. Zucker*
Affiliation:
U.S. Environmental Protection Agency, ORD, NHEERL
*
zucker.robert@epa.gov

Extract

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The confocal laser-scanning microscope (CLSM) has enormous potential in many biological fields. The reliability of the CLSM to obtain specific measurements and quantify fluorescence data is dependent on using a correctly aligned machine that contains a stable laser power. For many applications it is useful to know the CLSM system's performance prior to acquiring data images so the necessary resolution, sensitivity and precision can be obtained. Applications involving deconvolution, FRET and quantification necessitate that the confocal microscope is correctly configured and operating at the highest performance levels.

Type
Research Article
Information
Microscopy Today , Volume 10 , Issue 5 , September 2002 , pp. 8 - 13
Copyright
Copyright © Microscopy Society of America 2002

References

References:

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7. The research described in this article has been reviewed and approved for publication as an EPA document. Approval does not necessarily signify that the contents reflects the views and policies of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.Google Scholar
8. To whom all correspondence and reprint requests should be addressed: U.S. Environmental Protection Agency, Reproductive Toxicology Division (MD-72), National Health and Environmental Effects Research Laboratory, Research Triangle Park, NC 27711; Phone: (919) 541-1585; FAX: (919) 541-4017 email: Google Scholar