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Confocal Scanning Laser Holography: A Tool for Non-Invasive Internal Measurement

Published online by Cambridge University Press:  14 March 2018

RA McLeod ,
P Jacquemin ,
S Lai  and
RA Herring
RA McLeod*
Affiliation:
University of Victoria, British Columbia, Canada
P Jacquemin
Affiliation:
University of Victoria, British Columbia, Canada
S Lai
Affiliation:
University of Victoria, British Columbia, Canada
RA Herring
Affiliation:
University of Victoria, British Columbia, Canada
*
rmcleod@me.uvic.ca

Extract

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Confocal Holography is a combination of two well known concepts: confocal microscopy and light (laser) holography. Confocal microscopy places an aperture at a conjugate focus to the specimen focus. This filters any rays that are not on the focus plane, allowing a 3-dimensional image of the specimen to be built up over a set of planes.

Holography is the measurement of both the amplitude and phase characteristics of light. Typically most methods only measure the amplitude of the image. The phenomenon of interference allows the determination of the phase shift for a coherent source as well. The phase information is directly related to the index of refraction of a material, which in turn is a function of the temperature and composition.

Type
Research Article
Information
Microscopy Today , Volume 13 , Issue 1 , January 2005 , pp. 30 - 31
Copyright
Copyright © Microscopy Society of America 2005

References

RA Herring: Confocal scanning Laser holography, and an associated microscope: a proposal. Optik 105 No. 2 (1997) pp. 65–58.Google Scholar
Dixon, AE, Damaskinos, S, Atkison, MR: A scanning confocal microscope for transmission and reflection imaging. Nature 351 (1991) pp. 551–553.Google Scholar