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Cryostat Sectioning of Undecaicified Mouse Appendage Joints for use in Immunohistochemical Staining Procedures

Published online by Cambridge University Press:  14 March 2018

Timothy B. Plummer  and
Steven C. Ziesmer
Timothy B. Plummer
Affiliation:
Department of Laboratory Medicine and Pathology, Mayo Foundation
Steven C. Ziesmer
Affiliation:
Department of Laboratory Medicine and Pathology, Mayo Foundation

Extract

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Immunohistochemical analysis was required to identity lymphoid cell subtypes in mouse appendage joint synovium tissue. Hamster and rat monoclonal antibodies directed toward lymphoid cell subtypes were found to be nonreactive in formalin-fixed decalcified appendages, and this necessitated the use of cryostat sections for immunohistochemical procedures. Initial attempts to section mouse joints using conventional cryostat techniques did not yield adequate sections, and it was clear that a different approach was necessary.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 8 , October 1997 , pp. 18 - 19
Copyright
Copyright © Microscopy Society of America 1997

References

Lundin, D. Henry Ford Hospital. Detroit, MI. Personal communication to Timothy Plummer, October 1995.Google Scholar
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