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Electron Microscopy Analysis of Multilamellar Vesicles Prepared from Synthetic Lipids: A Model System for Studying Membrane Structure in the Molecular Cell Biology Classroom and Laboratory

Published online by Cambridge University Press:  14 March 2018

Russell R. Camp ,
Jungsoo Byun  and
Robert Jacob
Russell R. Camp
Affiliation:
Gordon College, Wenham, MA
Jungsoo Byun*
Affiliation:
Gordon College, Wenham, MA
Robert Jacob
Affiliation:
Elucida Research, LLC, Beverly, MA USA
*
[jbyun@elucidaresearch.com]

Extract

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The cell is the fundamental unit of all living organisms, ranging from the unicellular archaea and bacteria (prokaryotes) to higher multicellular plants and animals (eukaryotes). All cells are bounded by a complex and dynamic plasma membrane, which functions principally to maintain cellular and organismal steady state by performing complex energy transformations and regulating the flow of information for the cell. The cell membrane also performs a number of vital housekeeping functions, which include control of the transport of substances between extracellular and intracellular environments, participation in cell signaling cascades by hosting receptors of extracellular ligands, and facilitating critical cell-to-cell communications in multicellular organisms (Karp, 2005).

Considerable research over the last fifty years has significantly increased our understanding of cell membranes and their structural organization. Every membrane is fundamentally comprised of a dynamic lipid bilayer that supports a variety of transmembrane and membraneassociated proteins.

Type
Research Article
Information
Microscopy Today , Volume 12 , Issue 5 , September 2004 , pp. 40 - 45
Copyright
Copyright © Microscopy Society of America 2004

References

Alberts, B., Lewis, J., Raff, M., Robert, K., and Watson, J.D. 2002. Molecular biology of the cell (4th edition). Garland: New York. 1463p.Google Scholar
Bangham, A. D. and Horne, R. W. 1964. Negative staining of phospholipids and their structural modification by surface-active agents as observed in the electron microscope. J Mol Biol 12:660-8.Google Scholar
Chatterjee, S. N. and Agarwal, S. 1988. Liposomes as membrane model for study of lipid peroxidation. Free radical biology and medicine, Vol 4, pp. 51–72.Google Scholar
Handjani-Vila, R.M., Ribier, A., and Vanlerberghe, G. 1993. Liposomes in the cosmetics industry. Chapter 12, p. 201–213. From Liposome Technology 2nd Edition Volume II. Ed. Gregoriadis, G. CRC Press, London.Google Scholar
Jacob, R. F., Cenedella, R. I., and Mason, R. P. 1999. Direct evidence for immiscible cholesterol domains in human ocular lens fiber ceil plasma membranes. J Biol Chem 274(44): 31613–31618.Google Scholar
Karp, G. 2005. Cell and molecular biology: concepts and experiments (4th edilion), John Wiley: New York. 780p.Google Scholar
Mozafari, M. R., Reed, C. J., Rostron, C., Kocum, C. and Piskin, F.. 2002. Construction of Stable Anionic Uposome-Plasmid Particles Using the Healing Method: A Preliminary Investigation, Cell Mol Biol Lett 7: 923–927.Google Scholar
Olson, F., Hunt, C.A., Szoka, F. C., Vail, W J., and Papahadjopoulos, D, 1979. Preparation of liposomes of defined distribution by extrusion through polycarbonate membranes. Biochimica et Biophysica Acta 557:9–23.Google Scholar
Perrett, S., Golding, M., and Williams, P. 1999. A simple method tor the preparation of liposomes for pharmaceutical applications: characterization of the liposomes. J. Pharm. Pharmacol. 43:154–161.CrossRefGoogle Scholar
Zimmer, A., Aziz, S.A., Gilbert, M., Werner, D. and Noe, C.R. 1999 Synthesis of Cholesterol Modified Cationic Lipids for Liposomal Drug Delivery of Antisense Oligonudeotides. Eur J Pharm Biopharm 47: 175–178.Google Scholar