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Fluorescence Resonance Energy Transfer Microscopy

Published online by Cambridge University Press:  14 March 2018

Melanie L. Stenz  and
Ammasi Periasamy
Melanie L. Stenz
Affiliation:
W.M. Keck Center for Cellular imaging, University of Virginia
Ammasi Periasamy*
Affiliation:
W.M. Keck Center for Cellular imaging, University of Virginia
*
E-mail: ap3t@virginia.edu

Extract

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Fluorescence is a property of certain fluorophores, which allows them to absorb light at one wavelength and emit the light at another, longer wavelength. This fluorescence can be used as a tool to study the interactions of numerous components within a cell. With the use of fluorescent probes, proteins or other cellular matter can be specifically labeled in a fixed or live cell. This allows scientists to closely monitor microscopic cellular functions in order to gain a greater understanding of living biological processes.

Fluorescence Resonance Energy Transfer (FRET) imaging microscopy is an important and reliable tool for studying minute molecular processes in space and time within a single cell. FRET spectroscopy is a better method for studying the structure and localization of proteins in physiological conditions than are X-ray diffraction, nuclear magnetic resonance, and electron microscopy.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 3 , April 2000 , pp. 10 - 13
Copyright
Copyright © Microscopy Society of America 2000

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