A Fool-Proof Method For Mounting Serial Sections On Single Hole Grids

Extract

I did serial sectioning for years on large single hole grids using a very simple technique that made the potential problems of film thickness, wrinkles and section loss very minor. I was not the original developer of the method and do not remember who originally gave it to me. It goes as follows:

• 1) Have your machine shop cut some thin pieces of Plexiglas into the size of glass slides. At one end, drill about a dozen holes, roughly 5 mm in diameter, in an area about the size of a formvar film cast on glass slides. These slides will serve as your template for holding your films.