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Published online by Cambridge University Press: 14 March 2018
In regards to the purchase of a histology diamond knife for preparing light microscopy sections, I was one of those folks, in possession of a nice glass knife maker, who was very comfortable with breaking, making and using glass knives. So the question was—did I really need a costly diamond knife for making light microscopy sections?
Once I finally tried a diamond histo knife, I never went back to glass. There are some very good incentives for this: long lasting cutting surface, much time saved in sample preparation, larger block faces can be prepared, and more consistent cutting results obtained. A histo diamond knife is also a big aid in preparing specimens for diagnostic or biological microscopy. These very important bonuses make a good list to have in hand when justifying the purchase to your organization. However, are they worth the price? Yes, I think so—even so far as providing each tech with their own diamond histo knife.
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