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High Pressure Freezing; Benefits and Limitations

Published online by Cambridge University Press:  14 March 2018

Robert R. Wise
Robert R. Wise*
Affiliation:
University of Wisconsin-Oshkosh

Extract

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Rapid freezing of biological samples for subsequent processing and examination in the TEM has been around since the 1960's. Freezing in general, is advantageous because it can provide an instantaneous cessation of all biological activity and hall ultrastructural rearrangement The freezing ‘fixes” biological structures in water ice until such time that the ice can be substituted for a chemical fixative. Freezing is typically conducted at liquid nitrogen temperatures (-196°C), and freeze substitution (FS) is then carried out over days to weeks on dry ice (-80°C) (Severs el. al., 1995).

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 9 , November 1998 , pp. 14 - 15
Copyright
Copyright © Microscopy Society of America 1998

References

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