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Home-Made Cell Tack

Published online by Cambridge University Press:  14 March 2018

Kristen R. Ross  and
Micheal E. Berens
Kristen R. Ross*
Affiliation:
Barrow Neurological Institute
Micheal E. Berens*
Affiliation:
Barrow Neurological Institute
*
kross@chw.edu
mberens@CHW.EDU

Extract

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We've used gelled agarose to cover & hold monolayer cells in place when treatment leads to cell lifting. Low-gelling temperature agarose (Type VII) (Sigma A 4018 or A 0701) can be made as a 2% stock solution in “phosphate-buffered saline” (PBS) (boiled then kept at 42° C to remain liquid).

Type
Microscopy 101
Information
Microscopy Today , Volume 8 , Issue 8 , October 2000 , pp. 36
Copyright
Copyright © Microscopy Society of America 2000