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μManager: Open Source Software for Light Microscope Imaging

Published online by Cambridge University Press:  14 March 2018

Nico Stuurman ,
Nenad Amdodaj  and
Ron Vale
Nico Stuurman*
Affiliation:
University of California, San Francisco, CA
Nenad Amdodaj
Affiliation:
University of California, San Francisco, CA
Ron Vale
Affiliation:
University of California, San Francisco, CA
*
nico@cmpmail.ucsf.edu

Extract

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No longer are biologists content to peer through the oculars of a microscope. Eyes have been replaced by digital cameras. Instead of manual control of microscope stages, filters and light sources, modern microscopes have become fully robotic. Image acquisition and robotic movements require computer control. With such equipment it is possible to automatically record images at multiple wavelengths, carry out time-lapse recordings of living cells using very short exposure times and low light doses, and even to record images at multiple positions (e.g. from a multi-well plate) without operator intervention. Clearly, computer control of light microscope image acquisition is making many new experimental imaging strategies possible in the biological sciences.

Type
Research Article
Information
Microscopy Today , Volume 15 , Issue 3 , May 2007 , pp. 42 - 43
Copyright
Copyright © Microscopy Society of America 2007