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Microparticles/Exosomes: Isolation and TEM Analysis

Published online by Cambridge University Press:  14 March 2018

Natalie Bauer ,
Jyoti Rai ,
Hairu Chen ,
Lillianne Harris ,
Lalita Shevde ,
Tim Moore  and
Judy King
Natalie Bauer
Affiliation:
Center for Lung Biology Department of Pharmacology
Jyoti Rai
Affiliation:
Center for Lung Biology
Hairu Chen
Affiliation:
Center for Lung Biology
Lillianne Harris
Affiliation:
Mitchell Cancer Institute
Lalita Shevde
Affiliation:
Mitchell Cancer Institute
Tim Moore
Affiliation:
Center for Lung Biology Department of Medicine
Judy King*
Affiliation:
Center for Lung Biology Department of Pharmacology Department of Pathology, University of South Alabama, Mobile, Alabama
*
jking@usouthal.edu

Extract

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Microparticles and exosomes are small vesicular fragments of cell membrane which are released from activated and apoptotic cells. Microparticles (MPs) range in size from 0.5-1.5 μm, and exosomes are 0.5 μm and under. For the purposes of this article we will refer to both categories as microparticles. They differ from apoptotic bodies based on their smaller size, intact structure, and lack of degraded nuclear material. MPs have been shown to be released from a variety of cell types including platelets, endothelium, vascular smooth muscle cells, dendritic cells, and tumor cells. Jimenez and others have shown that based on the stimulus and cell type the MPs released are both quantitatively and phenotypically distinct. More recent data have shown the proteomics of MPs released from human umbilical vein endothelial cells differ dependent on whether they are stimulated with PAI or TNF-α.

Type
Research Article
Information
Microscopy Today , Volume 17 , Issue 2 , March 2009 , pp. 42 - 45
Copyright
Copyright © Microscopy Society of America 2009

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