Microwave Immunohistochemistry On Mohs Cryostat Sections

Extract

With the advent of Mohs surgery in the dermatology clinics, making sure the margins are clear of any cancer is of the utmost importance to both patient and dermatopathologist. While the patient is stiil in the surgery suite, immunohistochemistry staining can be done in the laboratory microwave (Pelco microwave with waiter load cooler wattage controller arid temperature probe). The immunohistochemistry procedure takes only 25-30 minutes as compared to the more conventional methods that take 2 hours or more.

The microwave needs to be checked for hot spots using the Pelco #36140 microwave bulb array. Make sure the area in which you are going to put your slides has no hot spots, indicated by no illuminating bulbs. Using a Sigma Diagnostics #H6644 Humid Chamber; place a paper towel in the bottom of the chamber and fill with 1/4 inch of water.