Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-29T04:45:52.980Z Has data issue: false hasContentIssue false

Novel Use of Fluorescence Illumination with an Infrared Microscope

Published online by Cambridge University Press:  14 March 2018

Thomas J. Tague Jr.*
Affiliation:
Spectra-Tech, Inc.
Lisa M. Miller
Affiliation:
National Synchroton Light Source

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

It has become increasingly obvious that infrared microspectroscopy can be the analysis tool of choice when determining the chemical composition of biological and biomedical samples. Frequently, fluorescence illumination is required for sample characterization, which previously required the use of a separate optical microscope. There has also been a need in the semiconductor manufacturing industry for a single tool for visualizing particle contaminants on integrated wafers as well as the ability to chemically determine their nature. There is now a single microscope platform for conducting rapid Nomarski differential interference contrast and fluorescence illumination sample visualization as well as infrared analysis. This novel infrared microscope has applicability to many fields of investigation, including pharmacology, forensics, cell biology, histology, gemology, and geology.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2000

References

1. Schiering, D.W., Tague, T.J. Jr., Reffner, J.A., and Vogel, S.H., “A Dual Confocal Aperturing Microscope for IR Microscopy”, Analysis, in press.Google Scholar
2. Reffner, J.A. and Vogel, S.H., Confocal Microspect.romet System, US Patent 5,864,139, 1999.Google Scholar
3. Brumberg, E.M., “Fluorescence Microscopy of Biological Objects Using Light From Above”, Biophysics, (4) 1959.Google Scholar
4. Kalasinsky, K.S., Cellular and Molecular Biology, Vol 44(1)1998, pp. 8187.Google Scholar
5. Wetzel, D.L. and LeVine, S.M., Science, Vol. 285 (5431), 1999, pp. 12241225.Google Scholar
6. Miller, L.M., Tibrewala, J., and Carlson, C.S., “Examination of Bone Chemical Composition in Osteopo- rosis Using Fluorescence - Assisted Synchrotron IR Mi croscopy”, Cellular Biology, in press.Google Scholar
7. Miller, L.M., Huang, R., Chance, M.R., and Carlson, C.S., “Applications of Fluorescence-Assisted Infrared Microspectroscopy to the Study of Osteoporosis”, Synchrotron Radiation News, Vol. 12 (1), 1999, pp. 2128.Google Scholar
8. Torzilli, P.A., Brustein, A.H., Takebe, K., Zika, J.C.. and Heiple, K.G., “The Mechanical and Structural Properties of Maturing Bone”, Mechanical Properties of Bone, American Society of Mechanical Engineers, New York, 1981, pp. 145161.Google Scholar
9. Fogarassy, E., Slaoui, A., Fuchs, C, and Regolini, J.L., Appl. Phys.Lett., Vol. 51 337, 1987.CrossRefGoogle Scholar
10. Chyan, O.M.R., Wu, J., and Chen, J.J., Appl. Spec, Vol. 51 (12) 1997, pp. 19051909.CrossRefGoogle Scholar