Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-27T08:42:38.289Z Has data issue: false hasContentIssue false
  • English
  • Français

Optimizing the Sampling Design of Morphometric Experiments

Published online by Cambridge University Press:  14 March 2018

John M. Basgen
John M. Basgen*
Affiliation:
University of Minnesota
*
basgen@umn.edu

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In a previous article [1], stereological methods for measuring volume, surface, length, and number were described. The present article will briefly discuss sampling methods, as well as techniques for optimizing the number of animals per group and the number of measurements per animal when planning a morphometric study.

Type
Research Article
Information
Microscopy Today , Volume 11 , Issue 3 , June 2003 , pp. 5 - 9
Copyright
Copyright © Microscopy Society of America 2003

References

1. Basgen, JM. (2002) Basic stereology. Microscopy Today 11 (1): 12–16.CrossRefGoogle Scholar
2. Stuart, A. (1976) Basic Ideas of Scientific Sampling, Second Edition, Charles Griffin amp; Co.r London, pages 10–11.Google Scholar
3. Weibel, ER. (1979) Stereological Methods Practical Methods for Biological Morphometry, Academic Press, New York, pages 82—83.Google Scholar
4. Gundersen, HJG. (1986) Stereology of arbitrary particles. Journal of Microscopy 143:3-45.CrossRefGoogle Scholar
5. Rosner, B. (1982) Fundamental of Biostatistics, Duxbury Press, Boston pp, 189–194.Google Scholar
6. Gundersen, HJG and ∅sterby R. (1981) Optimizing sampling efficiency of stereological studies in biology: or “Do more less well!”. Journal of Microscopy 121:65-73.Google Scholar
7. Gupta, M, Mayhew, TM, etai. (1983) Inter-anima! variation and its influence on the overall precision of morphometric estimates based on nested sampling design. Journal of Microscopy 131:147-154.Google Scholar
8. Mayhew, TM. (1933) Stereology: progress in quantitative microscopical anatomy. Progress in Anatomy Volume 3, Editors: V Navaratnam and RJ Harrison pp. 81-112.Google Scholar
9. Howard, CV and Reed, MG, (1998) Unbiased Stereology: Three-Dimensional Measurement in Microscopy, Bios-Springer Publishing, New York, pp.163–165.Google Scholar
10. Cruz-Orive, L. (1994) Toward a more objective biology. Neurobioiogy of Aging 15:377-378.CrossRefGoogle Scholar
11. Weibel, ER. (1979) Stereological Methods Practical Methods for Biological Morphometry, Academic Press, New York, pp. 110–116.Google Scholar
12. Cruz-Orive, LM Weibel, ER (1990) Recent stereological methods for cell biology: a brief survey, American Journal of Physiology 258(4pt1):L148-156.Google Scholar