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Preparing Diatoms for EM

Published online by Cambridge University Press:  14 March 2018

Greg Strout
Greg Strout*
Affiliation:
University of Oklahoma
*
gstrout@ou.edu

Extract

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Diatoms make for beautiful specimens for both transmission and scanning electron microscopy. As well, they are studied by many people, and there is always a need for good diatom preparations for EM.

I find that the diatoms in diatomaceous earth are usually broken, and like to prepare my own from fresh specimens. The materials needed for this are a plankton net, some potassium dichromate, and 30% hydrogen peroxide. Once the diatoms are collected (plankton net tows from shoreline, or wherever), they can be cleaned using the chemicals.

Type
Microscopy 101
Information
Microscopy Today , Volume 8 , Issue 9 , November 2000 , pp. 36 - 37
Copyright
Copyright © Microscopy Society of America 2000