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Probing Ribosomal RNA By Electron Spectroscopic Imaging and Three-Dimensional Reconstruction

Published online by Cambridge University Press:  14 March 2018

Daniel R. Beniac ,
Gregory J. Czarnota ,
Brenda L. Rutherford ,
F. Peter Ottensmeyer  and
George Harauz
Daniel R. Beniac
Affiliation:
Department of Molecular Biology and Genetics, and Biophysics Interdisciplinary Group, University of Guelph
Gregory J. Czarnota
Affiliation:
Molecular and Structural Biology, Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto
Brenda L. Rutherford
Affiliation:
Molecular and Structural Biology, Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto
F. Peter Ottensmeyer
Affiliation:
Molecular and Structural Biology, Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto
George Harauz
Affiliation:
Department of Molecular Biology and Genetics, and Biophysics Interdisciplinary Group, University of Guelph

Extract

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The ribosome is the protein synthetic machinery in the cell. Knowledge of the structures of ribosomal RNA (rRNA) macromolecules in situ is essential to understanding their roles in ribosome mediated protein synthesis. We are using a microanalytical technique that identifies and maps elements directly, electron spectroscopic imaging, to determine the rRNA phosphorus distributions within Escherichia coli ribosomal subunits, and to combine the two-dimensional maps into a three-dimensional elemental distribution by iterative quaternion-assisted angular reconstitution of ribosomal particles at random orientations.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 1 , January 1997 , pp. 10 - 11
Copyright
Copyright © Microscopy Society of America 1997

References

1 Bauer, R (1988) MeSh. Mierobiol. 20,113-146.Google Scholar
2 Bazett-Jones, DP (1993) Microbeam Anal. J. 2, 69-79.Google Scholar
3Bazett-Jones DP, Leblanc B, Herfort M, Moss T (1994) Science 264, 1134-1137.CrossRefGoogle Scholar
4 Bazett-Jones, DP, Mendez, E, Czarnota, GJ, Ottensmeyer, FP, Allfrey, VG (1996) Nucl. Acids Res . 24, 321–329.CrossRefGoogle Scholar
5Bazett-Jones DP, Oltensmeyer FP (1981) Science 211,169-170.CrossRefGoogle Scholar
6Beniac DR, Hsrauz G (1995) M6I. Cell. Biocnem. 148,165-181Google Scholar
7 Boublik, M, Oostergetel, GT, Frankland, B, Ottensmeyer, FP (1984) In Bailey GW (ed.), Proceedings of the 42nd Annual Meeting of the Electron Microscopy Society of America. San Francisco Press, San Francisco, pp 690–691.Google Scholar
8 Boublik, M, Mandiyan, V, Tumminia, S, Hainfeld, JF, Wall, JS (1990) In Proceedings of the Xllth International Congress for Electron Microscopy. San Francisco Press, San Francisco, pp 134–135.Google Scholar
9 Boublik, U, Wall, J (1992) In Bailey, GW, Bentley, J, Small JA (feds.). Proceedings of the 50fh Annual Meeting of the Electron Microscopy Society of America. San Francisco Press, San Francisco, pp 462–463.Google Scholar
10Czarnota GJ, Ottensmeyer FP (1996) J. Biol. Chem. 271: 3677-3633.Google Scholar
11Farrow NA, Ottensmeyer FP (1S93) Ultra microscopy 52,141-156, 1993.CrossRefGoogle Scholar
12Frank J, Zhu J; Penczek P, Li Y, Srivastava S, Verschoor A, Radermactier M, Grassucci R, Lafa RK, Agrawal RK (1995) Nature 376, 441-444.CrossRefGoogle Scholar
13 Korn, AP Spitnik-Elson, P, Elson, D, Ottensmeyer, FP (1983) Eur. J. Cell Biol, 31, 334–340.Google Scholar
14Ottensmeyer FP (1982) Science 215, 461-466.CrossRefGoogle Scholar