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Resolution Considerations for Photomacrography and Photomicroscopy

Published online by Cambridge University Press:  14 March 2018

Theodore M. Clarke
Theodore M. Clarke*
Affiliation:
Case Corporation

Extract

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My Inter/Micro-84 presentation and subsequent article in The Microscope dealt with optimizing diffraction limited depth of field in photomacrography and is referenced by Brian Bracegirdle in his “Scientific Photomacrography. My analysis was based upon the classical solution for the diffraction pattern image of a point light source, using the Rayleigh criterion of resolution. H. Lou Gibson's method of treating combined diffraction and geometric blurring away from the object focal plane was used. The calculations and the experimental results indicated that a final print resolution, for the object focal plane, of 7 lines/mm (.29 mm Airy disk) gives an optimum balance of depth of field and resolution. The 7 lines/mm criterion is equivalent to an Abbe criterion of total magnification equal to 440 times the numerical aperture. The 500 times numerical aperture Abbe criterion recommended for microscopists with very acute vision corresponds to a maximum resolution of 6 lines/mm in the final image.

Type
Research Article
Information
Microscopy Today , Volume 4 , Issue 4 , May 1996 , pp. 10 - 11
Copyright
Copyright © Microscopy Society of America 1996

References

1. Clarke, T. M., “Method for Calculating Relative Apertures for Optimizing Diffraction-Limited Depth of Field in Photo macrography”, Microscope 32, 219-258 (1984).Google Scholar
2. Bracegirdle, Brian, “Scientific Photomacrography”, RMS Microscopy Handbook, Wo. 31, BIOS Scientific Publishers Ltd., Oxford, U.K. Google Scholar
3. Gibson, H. Lou, “Photomacrography. Mathematical Analses of Magnification and Depth of Detail”, Kodak Publication No N-15.Google Scholar
4. Shillaber, C.P., “Photomicrography in Theory and Practice”, John Wiley & Sons, Inc. (1944).Google Scholar
5. Gibson, H. Lou, “Deplh and Enlarging Factors in Ultra-Close-up and Fhofomacrographic Prints and Slides”, BPA 54, 127-142 (1986).Google Scholar
6. Clarke, T.M., Letter to the Editor, Microscope 35, 332-336 (1987).Google Scholar
7. Neblette, C.B. & Murray, A.E., “Photographic Lenses”, Morgan & Morgan, Inc. (1973).Google Scholar
8. Clarke, T.M., “Light Microscopy Criteria for Electronic Imaging”, Microscope 43:3, 121-124 (1995).Google Scholar