Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-27T08:13:04.544Z Has data issue: false hasContentIssue false
  • English
  • Français

The Use of Ultrarapid Freezing and Freeze Substitution to Verify Vitrification and/or Ice Formation in Vascular Tissue

Published online by Cambridge University Press:  14 March 2018

Fred Lightfoot ,
Michael Taylor ,
Kelvin G.M. Brockbank  and
Cindy Hastings
Fred Lightfoot*
Affiliation:
Organ Recovery Systems, Inc.
Michael Taylor
Affiliation:
Organ Recovery Systems, Inc.
Kelvin G.M. Brockbank
Affiliation:
Organ Recovery Systems, Inc.
Cindy Hastings
Affiliation:
Central Arkansas Veterans Healthcare System
*
*flightfoot@organ-recovery.com

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The expanding field of cryobiology, in particular that of the study of vitrification for long term storage of tissues for transplantation, has demonstrated that ice is damaging to smooth muscle tissue. Consequently conventional methods such as ultrarapid freezing and freeze substitution are becoming routine protocols to determine the quality of cryopreservation. This article introduces the scientific community to the PS-1000 cryofixation unit (Delaware Diamond Knives, Wilmington, DE) which provides both ultrarapid freezing and a means of validation of freeze substitution methods. When any tissue is frozen or vitrif ed for clinical use it is imperative to know the structural and functional integrity of these tissues. Ice formation within the extracellular matrix and dehydration of multi-cellular tissues, using conventional cryopreservation, is the principal reason why these methods frequently prove to be ineffective.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 9 , November 2000 , pp. 16 - 21
Copyright
Copyright © Microscopy Society of America 2000

References

1. Mazur, P. (1984), and Freezing of living cells: mechanisms and implications, Am. J. of Physiology, vol.247, no. Ceil Physiol 16, p. C125–C142.Google Scholar
2. Taylor, M.J. (1984), Sub-zero preservation and the prospect of long-term storage of multicellular tissues and organs, Transplantation immunology: clinical and experimental, Calne, R.Y., ed., Oxford University press, Oxford News York Tokyo, pp. 360–390.Google Scholar
3. Dubochet, J. (1984), Electron microscopy of vitrified specimens, Proc. 8th Eur. Conf. Electron Microscopy, Budapest 2, 1379.Google Scholar
4. Franks, F. (1977), Biological freezing and cryofixation, J. Microscopy 111,3.Google Scholar
5. Feder, N. (1958), Methods and Principles of Fixation by Freeze-Substitution, J. Biophysic and Biochem Cytol., 1958, Vol. 4. No. 5.Google Scholar
6. Song, Y., (2000), Vitreous Cryopreservation Maintains the Function of Vascular Grafts, Nature Biotechnology, Vol. 18, March 2000, pp. 296–299.Google Scholar
7. Brockbank, K., (2000), Interstitial Ice Formation in cryopreserved Homografts: A Possible Cause of Tissue Deterioration and Calcification In Vivo, J. Heart Valve Disease, Vol. 9, No.2, March 2000, pp. 200–206.Google Scholar