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Virus Identification by Electron Microscopy

Published online by Cambridge University Press:  14 March 2018

Sara E. Miller
Sara E. Miller*
Affiliation:
Duke University Medical Center
*
saram@duke.edu

Extract

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Electron microscopy is clearly the best way to look at enteric viruses, many of which do not grow in tissue culture, and those that can be so coaxed, do so under special conditions that are not routinely found in the culture lab. Biochemical identification (e.g., immunological kits, PCR, Western Blots) require a specific reagent to recognize the virus, and if the right reagent is not used, the viruses will be missed (e.g., if you run a test for rotavirus, you will miss adenovirus, etc.). Furthermore, there are not biochemical reagents for all viruses. With electron microscopy, one can see a wide variety of viruses by doing a negative stain of an aqueous extract of stool.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 5 , June 2000 , pp. 28 - 29
Copyright
Copyright © Microscopy Society of America 2000

References

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