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Watching Rafts Move Within Cells: A Fluorescence Microscope-Based Transport Assay

Published online by Cambridge University Press:  14 March 2018

Stephen W. Carmichael  and
Jeffery L. Salisbury
Stephen W. Carmichael
Affiliation:
Mayo Clinic
Jeffery L. Salisbury
Affiliation:
Mayo Clinic

Extract

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Imagine a raft in a canal between point A and point B. On that raft is a visible (fluorescent) cargo. Also, attached to that raft is a motor that will propel the raft only from A to B (anterograde transport). When the raft gets to point B, another motor is attached that can propel the raft, and its cargo, and the anterograde motor, back to point A (retrograde transport). Within a cell, the canals are microtubLiles, and a lot is known about anterograde and retrograde transport in some systems, but these phenomena have not been directly observed in a living, intact animal. Until now, that is. In a pair of very interesting papers, the laboratory of Jonathan Scholey has shown us convincing micrographs of anterograde and retrograde transport in an important animal model.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 1 , January 2000 , pp. 3 - 7
Copyright
Copyright © Microscopy Society of America 2000

References

Orozco, J.T., Wedaman, K.P., Signor, D., Brown, H., Rose, L., and Scholey, J.M., Movement of motor and cargo along cilia, Nature 398:674, 1999.CrossRefGoogle ScholarPubMed
Signor, D., Wedaman, K.P., Orozco, J.T., Dwyer, N.D., Bargmann, C.I., Rose, L. S., and Scholey, J.M., Role of class DHC1b dynein in retrograde transport of IFT motors and [FT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans, J. Cell Biol. 147:519-530, 1999. Video images from this study can be accessed at http://www.mcb.ucdavis.edu/faculty-labs/scholey/CrossRefGoogle Scholar