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Wide Field and Deep Focus imaging in Photomicrography Optical and Software-Based Techniques

Published online by Cambridge University Press:  14 March 2018

Jörg Piper
Jörg Piper*
Affiliation:
Clinic Meduna, Bad Bertrich, Germany
*
jpu.mp@t-online.de

Extract

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In photomicrography, two fundamental problems often arise in practice: The higher the magnification and the higher the thickness or three dimensional extension of the specimen, the lower is the depth of focus which can result in parts of the specimen being indistinct. The larger the area of the specimen, the lower is the resolution in images showing the specimen in a total view. Additional problems will occur, when the lowest magnification of the respective microscope is not low enough for inclusion of the specimen’s total size so that only parts of the specimen will be visible in photo micrographic images. Table 1 presents the focal depth and lateral resolution of microscopic objectives with regard to their magnification and numerical aperture (modified from [4] and [7]).

Type
Research Article
Information
Microscopy Today , Volume 16 , Issue 2 , March 2008 , pp. 32 - 37
Copyright
Copyright © Microscopy Society of America 2008

References

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