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Selective Cytotoxicities of Red-Allotrope Selenium Nanoparticles and Polyethylene Glycol Towards Head and Neck Squamous Cell Carcinoma in Comparison to Human Dermal Fibroblasts

Published online by Cambridge University Press:  10 May 2016

Christopher Hassan  and
Thomas J. Webster
Christopher Hassan*
Affiliation:
Department of Chemical Engineering, Northeastern University, Boston, MA 02115
Thomas J. Webster*
Affiliation:
Department of Chemical Engineering, Northeastern University, Boston, MA 02115 Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia
*
*(Email: hassan.christopher@gmail.com)
†Contact Author: Thomas J. Webster th.webster@neu.edu 617-373-2989
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Abstract

Given their low toxicity and natural presence in the human diet, selenium nanoparticles have been established as potential candidates for the treatment of numerous cancers. In this study, red-allotrope selenium nanoparticles (rSeNPs) were synthesized and characterized. Head and neck squamous cell carcinoma (HNSCC) and human dermal fibroblast (HDF) cell lines were cultured and exposed to rSeNPs at concentrations ranging from 0.01 to 100μg rSeNP/mL media for one to three days. The cytotoxicity of rSeNP towards HNSCC and HDF was analyzed. Results indicated that the particles were approximately four times as cytotoxic toward HNSCC compared to HDF, with their respective IC50 values at 19.22 and 59.61μg rSeNP/mL media. Using statistical analysis, an effective dosage range for killing HNSCC cells while simultaneously minimizing damage to HDF over a three-day incubation was established as 20 to 55μg rSeNP/mL media. Such results indicate that rSeNPs are a potential option for treating HNSCC. Due to its ability to minimize nanoparticle agglomeration, making a it a candidate with which to functionalize selenium, similar assays were performed using poly(ethylene glycol) with a molecular weight of 200g/mol (PEG200), with results indicating that only a slight cytotoxic effect exists for HNSCC and almost no effect for HDF.

Keywords

nanoscalebiologicalbiomedical
Type
Articles
Information
MRS Advances , Volume 1 , Issue 56: Biomaterials and Soft Materials/Engaged Learning of Materials Science and Engineering in the 21st Century , 2016 , pp. 3775 - 3782
Copyright
Copyright © Materials Research Society 2016 

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References

Stewart, Bernard W and Kleihues, Paul. World Cancer Report . International Agency for Research on Cancer, 2003: 232 – 6.Google Scholar
Carvalho, Andre Lopes, Nishimoto, Ines Nobuko, Califano, Joseph A, and Kowalski, Luiz Paulo. Trend in Incidence and Prognosis for Head and Neck Cancer in the United States: A Site-Specific Analysis of the SEER Database . International Journal of Cancer, 2004, 114: 806 – 816.Google Scholar
Koch, Wayne M, Brennan, Joseph A, Zahurak, Marianna, Goodman, Steven N, Westra, William H, Schwab, Donna, Yoo, George H, Lee, Ding Jen, Forastiere, Arlene A, Sidransky, David. p53 Mutation and Locoregional Treatment Failure in Head and Neck Squamous Cell Carcinoma . Journal of the National Cancer Institute, Nov 6, 1996, 88(21), 1580 – 1586.CrossRefGoogle ScholarPubMed
Forastiere, Arlene, Koch, Wayne, Trotti, Andrew, and Sidransky, David. Head and Neck Cancer . The New England Journal of Medicine, 2001, 345, 1890 – 1900.Google Scholar
Herrero, R. Human Papillomavirus and Cancer of the Upper Aerodigestive Tract . Journal of the National Cancer Institute Monographs, 2003, 47 – 51.Google Scholar
Reed, Andre L, Califano, Joseph, Cairns, Paul, Westra, William H, Jones, Richard M, Koch, Wayne, Ahrendt, Steven, Eby, Yolanda, Sewell, Duane, Nawroz, Homaira, Bartek, Jiri, and Sidranksy, David. High Frequency of p16 (CDKN2/MTS-1/INK4A) Inactivation in Head and Neck Squamous Cell Carcinoma . Cancer Research, Aug 15, 1996, 56, 3630 – 3633.Google Scholar
Clarke, A R, Purdie, C. A., Harrison, D. J., Morris, R. G., Bird, C. C., Hooper, M. L., and Wyllie, A. H.. Thymocyte Apoptosis Induced by p53-Dependent and Independent Pathways . Nature, Apr 29, 1993, 362, 849 – 852.Google Scholar
Wynford, Thomas D. p53 in Tumor Pathology: Can We Trust Immunocytochemistry? Journal of Pathology, Apr, 1992, 166(4), 329 – 330.CrossRefGoogle Scholar
Smith, Theodore A. G, Mehaffey, Michele G., Kayda, Dawn B., Saunders, June M., Yei, Soopin, Trapnell, Bruce C., McClelland, Alan, and Kaleko, Michael. Adenovirus Mediated Expression of Therapeutic Plasma Levels of Human Factor IX in Mice . Nature Genetics, 1993, 5, 397 – 402.Google Scholar
O’Donnell, Rebekah K, Kupferman, Michael, Jack Wei, S, Singhal, Sunil, Weber, Randal, O’Malley, Bert Jr, Cheng, Yi, Putt, Mary, Feldman, Michael, Ziober, Barry, and Muschel, Ruth J. Gene Expression Signature Predicts Lymphatic Metastasis in Squamous Cell Carcinoma of the Oral Cavity . Oncogene, 2005, 24, 1244 – 1251.Google Scholar
Shuming, Nie, Xing, Yun, Kim, Gloria J, and Simons, Jonathan W.. Nanotechnology Applications in Cancer . Annual Review of Biomedical Engineering, Aug 2007, 9, 257 – 288.Google Scholar
Wang, Qi and Webster, Thomas J. Short Communication: Inhibiting Biofilm Formation on Paper Towels through the Use of Selenium Nanoparticles Coatings . International Journal of Nanomedicine, 2013, 8, 407 – 411.Google Scholar
Tran, Phong A, Sarin, Love, Hurt, Robert H and Webster, Thomas J. Differential Effects of Nanoselenium Doping on Health and Cancerous Osteoblasts in Coculture on Titanium . International Journal of Nanomedicine, 2010, 5, 351 – 358.Google Scholar
Zheng, Shanyuan, Li, Xiaoling, Zhang, Yibo, Xie, Qiang, Wong, Yum-Shing, Zheng, Wenjie, and Chen, Tianfeng. PEG-Nanolized Ultrasmall Selenium Nanoparticles Overcome Drug Resistance in Hepatocellular Carcinoma HepG2 Cells through Induction of Mitochondria Dysfunction . International Journal of Nanomedicine, 2012, 7, 3939 – 3949.Google Scholar
Kim, Ryngsa. Recent Advances in Understanding the Cell Death Pathways Activated by Anticancer Therapy . Cancer, Apr 15, 2005, 103(8), 1551 – 1560.Google Scholar