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Upconversion Luminescence Imaging of Tumors with EGFR-Affibody Conjugated Nanophosphors

Published online by Cambridge University Press:  20 May 2019

Majid Badieirostami ,
Colin Carpenter ,
Guillem Pratx ,
Lei Xing  and
Conroy Sun Open the ORCID record for Conroy Sun [Opens in a new window]
Majid Badieirostami
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Colin Carpenter
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Guillem Pratx
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Lei Xing
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Conroy Sun*
Affiliation:
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR97201 Department of Radiation Medicine, School of Medicine, Oregon Health & Science University, Portland, Oregon97239
*
*(Email: sunc@ohsu.edu)
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Abstract

Near infrared (NIR) optical imaging has demonstrated significant potential as an effective modality for cancer molecular imaging. Among various NIR probes currently under investigation, upconversion nanophosphors (UCNPs) possess great promise due to their anti-Stokes emission and sequential photon absorption which result in superior detection sensitivity and a simple imaging setup, respectively. Here we investigated the utility of this imaging modality to detect tumor cells expressing the epidermal growth factor receptor (EGFR) using affibody functionalized nanophosphors and a custom built imaging system. Initially, aqueous dispersible NaYF4: Tm+3, Yb+3 UCNPs were synthesized and their photophysical properties were characterized. Then, their luminescence response as a function of concentration and their depth resolving capability in a tissue-simulating phantom were examined. Finally, we demonstrated the use of bioconjugated UCNPs for imaging EGFR-expressing tumors both in vitro and in vivo. Our data suggests that NIR imaging with UCNPs may be useful for noninvasive imaging of tumors.

Keywords

Lanthanidenanoscalebiomaterial
Type
Articles
Information
MRS Advances , Volume 4 , Issue 46-47: Soft Materials and Biomaterials , 2019 , pp. 2461 - 2470
Copyright
Copyright © Materials Research Society 2019 

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References

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