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Compared Biocompatibility of ZnTiO3, ZnO and TiO2 Sol-Gel Films with Human Mesenchymal Stem Cells

Published online by Cambridge University Press:  15 February 2016

Rosalía Delgado Carrascón ,
Dario Gallach Pérez ,
Josefa.P. Carcía Ruiz  and
Miguel Manso Silvan
Rosalía Delgado Carrascón
Affiliation:
Departamento de Física Aplicada, Universidad Autónoma de Madrid, Madrid, 28049, Spain.
Dario Gallach Pérez
Affiliation:
Departamento de Física Aplicada, Universidad Autónoma de Madrid, Madrid, 28049, Spain.
Josefa.P. Carcía Ruiz
Affiliation:
Departamento de Biología Molecular, Universidad Autónoma de Madrid, Madrid, 28049, Spain.
Miguel Manso Silvan*
Affiliation:
Departamento de Física Aplicada, Universidad Autónoma de Madrid, Madrid, 28049, Spain.
*
*(Email: miguel.manso@uam.es)
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Abstract

The biomedical applications of ZnO are drastically limited by its intrinsic solubility, which shortens the stability and lifetime of devices. We show that the functionality of ZnO in human mesenchymal stem cell (hMSC) studies is limited due to poor cell adhesion. The sol-gel route has been employed to obtain zinc titanate thin films for their integration as surface protective layer on ZnO. These films were obtained from zinc acetate (ZnAc) and titanium isopropoxide (TIPT). So derived xerogels were dried and their thermal evolution studied by TGM-DTA to identify critical annealing temperatures. The evolution of the microstructure and composition of spun cast films was determined by XRD and FTIR. Organic and ionic byproducts were eliminated at T>300°C, which kickstarts a transformation of the amorphous materials into polycrystalline. Thin films consisted of the ZnTiO3 perovskite from annealing temperatures of 500°C. Cell adhesion on the synthesized samples (both amorphous and crystalline) was assayed by culturing hMSCs. Immunofluorescence images of actin cytoskeleton were obtained and proliferation studied using Ki67. Cell density, single cell area and proliferation rates on ZnTiO3 films were closer to control TiO2 surfaces than to ZnO films. Such behavior validates the short term biocompatibility of ZnTiO3 films and its potential use as surface layer for ZnO biomedical devices.

Keywords

biomedicalsol-gelthin film
Type
Articles
Information
MRS Advances , Volume 1 , Issue 11: Nanomaterials and Synthesis , 2016 , pp. 737 - 742
Copyright
Copyright © Materials Research Society 2016 

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References

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