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The use of magnetic nanoparticles for immobilization and recycling of enzymes

Published online by Cambridge University Press:  13 August 2018

Shirley Furtado ,
Mariana Brandes ,
Catalina Alamón ,
Santiago Botasini  and
Ana M.B. Cantera
Shirley Furtado
Affiliation:
Cátedra de Bioquímica /Dpto. de Biociencias/Facultad de Química/Universidad de la República, Montevideo, Uruguay Laboratorio de Enzimas Hidrolíticas/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Mariana Brandes
Affiliation:
Laboratorio de Biomateriales/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Catalina Alamón
Affiliation:
Laboratorio de Biomateriales/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Santiago Botasini*
Affiliation:
Laboratorio de Biomateriales/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
Ana M.B. Cantera
Affiliation:
Cátedra de Bioquímica /Dpto. de Biociencias/Facultad de Química/Universidad de la República, Montevideo, Uruguay Laboratorio de Enzimas Hidrolíticas/ Instituto de Química Biológica/Facultad de Ciencias/Universidad de la República, Montevideo, Uruguay
*
*(Email: sbotasini@fcien.edu.uy)
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Abstract

The use of nanostructured materials for enzyme immobilization is an active field of research due to its large surface area and the new emergent properties derived from its size. The present work is focused on the synthesis of magnetic nanoparticles for the adsorption of cysteine-proteolytic enzymes extracted from Bromelia antiacantha Bertol (Bromeliaceae) fruit. The results show that enzyme adsorption is highly dependent on the temperature and pH. The biocatalyst activity increased up to 40 %, once immobilized onto the magnetic nanoparticles. In addition, they can be recovered using a magnet allowing them to be reused up to 5 cycles with a marginal loss (5 %) of the initial activity.

Keywords

ferromagneticmagneticnanostructurecatalytic
Type
Articles
Information
MRS Advances , Volume 3 , Issue 61: Materials Science Young Researchers of Uruguay , 2018 , pp. 3581 - 3587
Copyright
Copyright © Materials Research Society 2018 

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