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Biosynthesis and Characterization of Bacterial Cellulose Produced by a Wild Strain of Acetobacter spp.

Published online by Cambridge University Press:  14 January 2013

Fatima Yassine ,
Michael Ibrahim ,
Maria Bassil ,
Ali Chokr ,
Anatoli Serghei ,
Antoine El Samrani ,
Mario El Tahchi  and
Gisele Boiteux
Fatima Yassine
Affiliation:
LBMI, Department of Physics, Lebanese University - Faculty of Sciences II, PO Box 90656 Jdeidet, Lebanon, email: gbmi@ul.edu.lb, Tel: +961 3 209688, Fax: +961 1 681553. IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Lyon 1 University, ISTIL Building, 15 Boulevard A. Latarget, 69 622 Villeurbanne Cedex, France,email: gisele.boiteux@univ-lyon1.fr,Tel: +33 4 72 44 85 64, Fax: +33 4 78 89 25 83. PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies- Lebanese University, PO Box 5, Hadath Campus, Beirut, Lebanon, email: antoineelsamrani@ul.edu.lb Tel: +961 5 470936, Fax.+ 961 5 463278.
Michael Ibrahim
Affiliation:
LBMI, Department of Physics, Lebanese University - Faculty of Sciences II, PO Box 90656 Jdeidet, Lebanon, email: gbmi@ul.edu.lb, Tel: +961 3 209688, Fax: +961 1 681553.
Maria Bassil
Affiliation:
LBMI, Department of Physics, Lebanese University - Faculty of Sciences II, PO Box 90656 Jdeidet, Lebanon, email: gbmi@ul.edu.lb, Tel: +961 3 209688, Fax: +961 1 681553.
Ali Chokr
Affiliation:
PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies- Lebanese University, PO Box 5, Hadath Campus, Beirut, Lebanon, email: antoineelsamrani@ul.edu.lb Tel: +961 5 470936, Fax.+ 961 5 463278. Laboratory of Microbiology, Department of Biology, Faculty of Sciences I, Lebanese University, Beirut, Lebanon, e-mail: alichokr@ul.edu.lb, Tel: +961 70 924383.
Anatoli Serghei
Affiliation:
IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Lyon 1 University, ISTIL Building, 15 Boulevard A. Latarget, 69 622 Villeurbanne Cedex, France,email: gisele.boiteux@univ-lyon1.fr,Tel: +33 4 72 44 85 64, Fax: +33 4 78 89 25 83.
Antoine El Samrani
Affiliation:
PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies- Lebanese University, PO Box 5, Hadath Campus, Beirut, Lebanon, email: antoineelsamrani@ul.edu.lb Tel: +961 5 470936, Fax.+ 961 5 463278.
Mario El Tahchi
Affiliation:
LBMI, Department of Physics, Lebanese University - Faculty of Sciences II, PO Box 90656 Jdeidet, Lebanon, email: gbmi@ul.edu.lb, Tel: +961 3 209688, Fax: +961 1 681553. PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies- Lebanese University, PO Box 5, Hadath Campus, Beirut, Lebanon, email: antoineelsamrani@ul.edu.lb Tel: +961 5 470936, Fax.+ 961 5 463278.
Gisele Boiteux
Affiliation:
IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Lyon 1 University, ISTIL Building, 15 Boulevard A. Latarget, 69 622 Villeurbanne Cedex, France,email: gisele.boiteux@univ-lyon1.fr,Tel: +33 4 72 44 85 64, Fax: +33 4 78 89 25 83.
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Abstract

Many advances in nanomaterials synthesis have been recorded during the last 30 years. Bacterial cellulose (BC) produced by bacteria belonging to the genera Acetobacter, Rhizobium, Agrobacterium, and Sarcina is acquiring major importance as one of many eco-friendly materials with great potential in the biomedical field. The shape of BC bulk is sensitive to the container shape and incubation conditions such as agitation, carbon source, rate of oxygenation, electromagnetic radiation, temperature, and pH. The challenge is to control the dimension and the final shape of biosynthesized cellulose, by the optimization of culture conditions. The production of 3D structures based on BC is important for many industrial and biomedical applications such as paper and textile industries, biological implants, burn dressing material, and scaffolds for tissue regeneration. In our work, wild strains of Acetobacter spp. were isolated from homemade vinegar then purified and used for cellulose production. Four media of different initial viscosity were used. Cultures were performed under static conditions at 29°C, in darkness. The dimensions and texture of obtained bacterial cellulose nanofibers were studied using scanning electron microscopy (SEM). X-ray diffraction (XRD) showed that the biosynthesized material has a cellulose I crystalline phase characterized by three crystal planes. fourrier transform infrared spectroscopy (FTIR) data confirmed the chemical nature of the fibers. Thermo-gravimetric analysis (TGA) showed that BC preserves a relatively superior non-degradable fraction compared to microcrystalline cellulose.

Keywords

biomaterialnanostructurethermogravimetric analysis (TGA)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 109 - 114
Copyright
Copyright © Materials Research Society 2013 

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