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Advanced Characterization Techniques for Nanoparticles for Cancer Research: Applications of SEM and NanoSIMS for Locating Au Nanoparticles in Cells

Published online by Cambridge University Press:  13 May 2013

Paul J Kempen ,
Chuck Hitzman ,
Laura S Sasportas ,
Sanjiv S Gambhir  and
Robert Sinclair
Paul J Kempen
Affiliation:
Department of Material Science and Engineering, Stanford University, 496 Lomita Mall, Stanford, CA 94305-4034 U.S.A.
Chuck Hitzman
Affiliation:
Stanford Nanocharacterization Laboratory, Stanford University, 476 Lomita Mall, Stanford, CA 94305-4035 U.S.A.
Laura S Sasportas
Affiliation:
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 318 Campus Drive, Stanford, CA 94305-5427 U.S.A.
Sanjiv S Gambhir
Affiliation:
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 318 Campus Drive, Stanford, CA 94305-5427 U.S.A.
Robert Sinclair
Affiliation:
Department of Material Science and Engineering, Stanford University, 496 Lomita Mall, Stanford, CA 94305-4034 U.S.A.
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Abstract

The ability of nano secondary ion mass spectrometry (NanoSIMS) to locate and analyze Raman active gold core nanoparticles (R-AuNPs) in a biological system is compared with the standard analysis using the scanning electron microscope (SEM). The same cell with R-AuNPs on and inside the macrophage was analyzed with both techniques to directly compare them. SEM analysis showed a large number of nanoparticles within the cell. Subsequent NanoSIMS analysis showed fewer R-AuNPs with lower spatial resolution. SEM was determined to be superior to NanoSIMS for the analysis of inorganic nanoparticles in complex biological systems.

Keywords

scanning electron microscopy (SEM)secondary ion mass spectroscopy (SIMS)biological
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium MM – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 157 - 163
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Zavaleta, C. L., Hartman, K. B., Miao, Z., James, M. L., Kempen, P., Thakor, A. S., Nielsen, C. H., Sinclair, R., Cheng, Z. and Gambhir, S. S., Small 7(15), 2232–2240 (2011).CrossRefGoogle Scholar
Devaraj, N. K., Keliher, E. J., Thurber, G. M., Nahrendorf, M. and Weissleder, R., Bioconjugate chemistry 20(2), 397–401 (2009).CrossRefGoogle Scholar
Liu, Z., Tabakman, S., Sherlock, S., Li, X., Chen, Z., Jiang, K., Fan, S. and Dai, H., Nano research 3(3), 222–233 (2010).CrossRefGoogle Scholar
Roussel, L. Y., Stokes, D. J., Gestmann, I., Darus, M. and , R. J. Young, presented at the Proc. SPIE, 2009 (unpublished).Google Scholar
Koh, A. L., Shachaf, C. M., Elchuri, S., Nolan, G. P. and Sinclair, R., Ultramicroscopy 109(1), 111–121 (2008).CrossRefGoogle Scholar
Reimer, L., Measurement Science and Technology 11(12), 1826 (2000).CrossRefGoogle Scholar
Zhou, W. and Wang, Z. L., Scanning microscopy for nanotechnology: techniques and applications. (Springer, 2006), pp. 1–40.Google Scholar
Wu, B. and Becker, J. S., International Journal of Mass Spectrometry 307(1–3), 112–122 (2011).CrossRefGoogle Scholar
Pett-Ridge, J. and Weber, P., in Microbial Systems Biology, edited by Navid, A. (Humana Press, 2012), Vol. 881, pp. 375–408.CrossRefGoogle Scholar
Wells, J., Kilburn, M. R., Shaw, J. A., Bartlett, C. A., Harvey, A. R., Dunlop, S. A. and Fitzgerald, M., Journal of Neuroscience Research 90(3), 606–618 (2012).CrossRefGoogle Scholar
Steinhauser, M. L., Bailey, A. P., Senyo, S. E., Guillermier, C., Perlstein, T. S., Gould, A. P., Lee, R. T. and Lechene, C. P., Nature (2012).Google Scholar
Kircher, M. F., de la Zerda, A., Jokerst, J. V., Zavaleta, C. L., Kempen, P. J., Mittra, E., Pitter, K., Huang, R., Campos, C., Habte, F., Sinclair, R., Brennan, C.W., Mellinghoff, I.K., Holland, E.C. and Gambhir, S.S., Nature Medicine 18(5), 829–834 (2012).CrossRefGoogle Scholar
Jokerst, J. V., Thangaraj, M., Kempen, P. J., Sinclair, R. and Gambhir, S. S., ACS Nano 6(7), 5920–5930 (2012).CrossRefGoogle Scholar
Keren, S., Zavaleta, C., Cheng, Z., De La Zerda, A., Gheysens, O. and Gambhir, S., Proceedings of the National Academy of Sciences 105(15), 5844–5849 (2008).CrossRefGoogle Scholar
Mulvaney, S. P., Musick, M. D., Keating, C. D. and Natan, M. J., Langmuir 19(11), 4784–4790 (2003).CrossRefGoogle Scholar
Kempen, P. J., Stanford University, 2012.Google Scholar
Dykstra, M. J. and Reuss, L. E., Biological electron microscopy: theory, techniques, and troubleshooting. (Springer, 2003), pp. 1–71.10.1007/978-1-4419-9244-4CrossRefGoogle Scholar