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Biomedical Applications of Gold Nanoparticles Functionalized Using Hetero-Bifunctional Poly(ethylene glycol) Spacer

Published online by Cambridge University Press:  01 February 2011

Wei Fu ,
Dinesh Shenoy ,
Jane Li ,
Curtis Crasto ,
Graham Jones ,
Charles Dimarzio ,
Srinivas Sridhar  and
Mansoor Amiji
Wei Fu
Affiliation:
Department of Physics, Northeastern University, Boston, MA 02115
Dinesh Shenoy
Affiliation:
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115
Jane Li
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115
Curtis Crasto
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115
Graham Jones
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115
Charles Dimarzio
Affiliation:
Department of Electrical and Computer Engineering and the Keck Microscope Facility, Northeastern University, Boston, MA 02115
Srinivas Sridhar
Affiliation:
Department of Physics, Northeastern University, Boston, MA 02115
Mansoor Amiji
Affiliation:
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115
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Abstract

To increase the targeting potential, circulation time, and the flexibility of surface-attached biomedically-relevant ligands on gold nanoparticles, hetero-bifunctional poly(ethylene glycol) (PEG, MW 1, 500) was synthesized having a thiol group on one terminus and a reactive functional group on the other. Coumarin, a model fluorescent dye, was conjugated to the PEG spacer and gold nanoparticles were modified with coumarin-PEG-thiol. Surface attachment of coumarin through the PEG spacer decreases the fluorescence quenching effect of gold nanoparticles. The results of cellular cytotoxicity and fluorescence confocal analyses showed that the PEG spacer modified nanoparticles were essentially non-toxic and could be efficiently internalized in the cells within one hour of incubation.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.4
Copyright
Copyright © Materials Research Society 2005

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References

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