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Cationic Gelatin as a Gene Carrier

Published online by Cambridge University Press:  21 February 2011

K. E. Brown ,
J. Bathon ,
C. H. Huang ,
R. Dalai  and
K. W. Leong
K. E. Brown
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
J. Bathon
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
C. H. Huang
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
R. Dalai
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
K. W. Leong
Affiliation:
Departments of Biomedical Engineering and MedicineThe Johns Hopkins University, Baltimore, MD 21205
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Abstract

Cationic gelatin was evaluated as a non-viral vector for cell transfection. We hypothesized that cationic gelatin would be a nontoxic alternative to already existing viral and non-viral cationic vectors. Cationic gelatin was synthesized by modifying gelatin with hexanediamine. Complexation of cationic gelatin with psv-β-gal plasmid caused an electrophoretic mobility shift of the plasmid. Cationic gelatin/DNA complexes were optimized in terms of transfection efficiency in CHODUK XB1 and COS 7 cell lines. Maximal gene expression for both cell types occurred in serum free medium with chloroquine (100 μM) at cationic gelatin/DNA ratios of approximately 2 and 7. In comparison with DEAE dextran, polylysine and Lipofectamine, cationic gelatin was the most efficient in transfecting COS 7 cells, with up to 18% cells transfected. In a dye reduction cytotoxicity assay, cationic gelatin caused < 5% of cells to become nonviable at a concentration of 100 μg/ml, while the other transfection reagents tested at the same concentration caused 25–100% of cell death. These results suggest that cationic gelatin holds promise as an effective vehicle for gene delivery to mammalian cells.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 394: Symposium Z – Polymers in Medicine and Pharmacy , 1995 , 61
Copyright
Copyright © Materials Research Society 1995

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