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Dielectric Relaxation and Dynamic Light Scattering Study of Liposome in the Aqueous Solution

Published online by Cambridge University Press:  01 February 2011

Shyamal Kumar Kundu ,
Song Gi Choe ,
Wataru Yamamoto ,
Rio Kita  and
Shin Yagihara
Shyamal Kumar Kundu
Affiliation:
sspskk@rediffmail.com, Tokai University, Department of physics, School of Science, 1117 Kitakaname, Hiratsuka, CA, 259-1292, Japan, +81-463-58-1211, +81-463-50-2013
Song Gi Choe
Affiliation:
6asnm018@keyaki.cc.u-tokai.ac.jp, Tokai University, Department of physics, Hiratsuka, 259-1292, Japan
Wataru Yamamoto
Affiliation:
6atrd016@keyaki.cc.u-tokai.ac.jp, Tokai University, Department of physics, Hiratsuka, 259-1292, Japan
Rio Kita
Affiliation:
rkita@keyaki.cc.u-tokai.ac.jp, Tokai University, Department of physics, Hiratsuka, 259-1292, Japan
Shin Yagihara
Affiliation:
yagihara@keyaki.cc.u-tokai.ac.jp, Tokai University, Department of Physics, Hiratsuka, 259-1292, Japan
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Abstract

The dynamic light scattering (DLS) has been used to investigate the diffusion behavior and the size distribution of liposome. DLS experiments show two classes of particles with their hydrodynamic radii being 4-12 nm and 66-80 nm. The numbers of particles associated with the two peaks are estimated by means of the scattering properties of the particles, which show that the overwhelming majority is big ones.

Broadband dielectric spectroscopy (BDS) has been used to investigate the dynamical structure of liposome in aqueous solution of water, which makes it possible to precisely explain the molecular mechanism, structures and various properties as a function of temperature and frequency. We have observed four relaxation processes, where two low frequency processes are commonly observed in charged macromolecules in aqueous solution, third process appeared in the MHz region due to the motion of ions, which is also related to the molecular motion of the lipid, and the high frequency process appeared about 20 GHz is due to free water surrounding liposome. Gel-LC phase transition has been described very accurately from the temperature-dependent shape parameter, β, obtained from the Cole-Cole fitting. The β value in LC phase is smaller than that in gel phase.

Keywords

dielectric propertiesdiffusionbiomaterial
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1019: Symposium FF – Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease , 2007 , 1019-FF04-08
Copyright
Copyright © Materials Research Society 2007

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