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The fabrication of self-assembling peptides into nanofiber scaffolds through molecular self-assembly

Published online by Cambridge University Press:  17 March 2011

Xiaojun Zhao ,
Jessica Dai  and
Shuguang Zhang
Xiaojun Zhao
Affiliation:
Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA Both authors contributed equally. Shuguang@mit.edu http://web.mit.edu/lms/www
Jessica Dai
Affiliation:
Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA Both authors contributed equally. Shuguang@mit.edu http://web.mit.edu/lms/www
Shuguang Zhang
Affiliation:
Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
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Abstract

We designed and fabricated a class of self-assembling peptides into nanofiber scaffolds. KLDL-12 has been shown to be a permissible nanofiber scaffold for chondrocytes in cartilage 3-D cell cultures. However, the biochemical, structural, and biophysical properties of KLDL-12 remain unclear. We show that KLDL-12 peptides form stable β-sheet structures at different pH values and that KLDL-12 and RIDI-12 self-assemble into nanofibers. The nanofiber length, though, is sensitive to pH changes. These results not only suggest the importance of electrostatic attraction or repulsion affecting the fiber lengths but also provide us with useful information for rational design and fabrication of peptide scaffolds.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 823: Symposium W – Biological and Bioinspired Materials and Devices , 2004 , W1.1/O1
Copyright
Copyright © Materials Research Society 2004

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