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Novel electroporation System for both Gram-negative and Gram-positive Bacteria Assisted by Multi-Walled Carbon Nanotubes

Published online by Cambridge University Press:  01 February 2011

M. Giersig ,
I. Firkowska ,
J. Trosczcynska ,
M. A. Correa Duarte  and
J. A Rojas-Chapana
M. Giersig*
Affiliation:
Center of Advanced European Studies and Research (CAESAR), Division Nanoparticle Technology Ludwig-Erhard-Allee 2, 53175 Bonn, Germany
I. Firkowska
Affiliation:
Center of Advanced European Studies and Research (CAESAR), Division Nanoparticle Technology Ludwig-Erhard-Allee 2, 53175 Bonn, Germany
J. Trosczcynska
Affiliation:
Center of Advanced European Studies and Research (CAESAR), Division Nanoparticle Technology Ludwig-Erhard-Allee 2, 53175 Bonn, Germany
M. A. Correa Duarte
Affiliation:
Center of Advanced European Studies and Research (CAESAR), Division Nanoparticle Technology Ludwig-Erhard-Allee 2, 53175 Bonn, Germany
J. A Rojas-Chapana
Affiliation:
Center of Advanced European Studies and Research (CAESAR), Division Nanoparticle Technology Ludwig-Erhard-Allee 2, 53175 Bonn, Germany
*
* Corresponding Author: giersig@caesar.de
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Abstract

Gram positive (Lactoccocus lactis) and Gram negative bacteria (Escherichia coli) were used to study the interaction of water-dispersible muti-walled carbon nanotubes (CNTs) with the bacterial cell envelope during microwave (MW) energy exposure. It was observed that the addition of a tiny amount of CNTs to a medium containing bacteria and subsequent exposure of the samples to MW, leads to an intimate contact between the CNT tips and the cell envelope. This phenomenon can be explained in terms of attractive forces between opposite charges of polar structures. Since CNTs under MW irradiation behave like electric dipoles, this would make it possible for the CNTs to target the cell surface without inducing changes in the cell shape and viability. Thus, the electrochemical properties of CNTs and their capillarity make them useful tools for cell manipulation, and therefore for the intracellular transport of drugs, dyes or biomolecules.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.21
Copyright
Copyright © Materials Research Society 2005

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References

REFERENCES

1. Nolkrantz, K., Farre, C., Brederlau, A., Karlsson, R. I., Brennan, C., Eriksson, P. S., Weber, S.G., Sandberg, M., and Orwar, O., Anal Chem. 73, 4469 (2001)Google Scholar
2. Olofsson, J., Nolkrantz, K., Ryttsen, F., Lambie, B. A., Weber, S.G., and Orwar, O., Curr Opin Biotechnol. 14, 29 (2003)Google Scholar
3. Rae, J. L., and Levis, R. A., Pflugers Arch. 443, 664 (2002)Google Scholar
4. Khine, M., Lau, A., Ionescu-Zanetti, C., Seo, J., and Lee, L. P., Lab on a Chip, 5, (2005) (Advance Article)Google Scholar
5. Li, W. Z., Wen, J. G., Tu, Y., and Ren, Z. F., Appl. Phys. A. 73, 259 (2001)Google Scholar
6. Liz-Marzán, L., Giersig, M., and Mulvaney, P. J., Chem. Commun. 6, 731 (1996)Google Scholar
7. Liz-Marzán, L., Giersig, M., and Mulvaney, P. J., Langmuir 12, 4329 (1996)Google Scholar
8. Samec, M., J. young investigators 9, (2003)Google Scholar
9. Wadhawan, A., Garrett, D., and Perez, J. M., Applied Physics Letters 83, 2683 (2003)Google Scholar
10. Chen, C. W., Lee, M. H., and Clark, S. J., Applied Surface Science, 228, 143 (2004)Google Scholar
11. Moon, P., and Spencer, D. E., “Field Theory for engineers”, ed. van Nostrand, D. (Princeton, NJ, 1961)Google Scholar
12. Rojas-Chapana, J. A, Correa-Duarte, M. A., Rhen, Z., Kempa, K., and Giersig, M., Nano Lett. 4, 985 (2004)Google Scholar
13. Correa-Duarte, M. A., Wagner, N., Rojas-Chapana, J. A., Morsczeck, C. O., Thie, M., and Giersig, M.. Nano lett. 4, 2233 (2004)Google Scholar